Thumm prac sandbox

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Cas9 is the RNA-guided DNA endonuclease used by the CRISPR (clustered regularly interspaced short palindromic repeats)-associated systems to generate double-strand DNA breaks in the invading DNA during an adaptive bacterial immune response.

The CRISPR-associated endonuclease Cas9 has been exploited for use in genome editing systems. In such systems, an engineered single-guide RNA (sgRNA) is used to target double-stranded breaks in genomic DNA. Depending on what repair pathway is triggered, often dictated by the inclusion of additional engineered components, the targeted site either is disrupted or incorporates additional genetic sequences.

Articles in Proteopedia concerning Cas9 include:

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Contents

3D structures of Cas9

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Drag the structure with the mouse to rotate

Streptococcus pyogenes Cas9

  • 4un3, 4un4, and 4un5 - S. pyogenes Cas9 bound to sgRNA and target DNA
  • 4oo8 - S. pyogenes Cas9 bound to sgRNA and target DNA
  • 4cmp
  • 4cmq - Mn2+-bound S. pyogenes Cas9

Actinomyces naeslundii Cas9

  • 4oge
  • 4ogc - Mn2+-bound A.s naeslundii Cas9

See Also

HNH endonuclease


References

Proteopedia Page Contributors and Editors (what is this?)

Brett M. Thumm

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