|Flow chart showing the major steps in X-ray protein crystallography. (Image from Wikimedia courtesy Thomas Splettstoesser.|
About 85% of the models (entries) in the World Wide Protein Data Bank were determined by X-ray crystallography. (Most of the remaining 15% were determined by solution nuclear magnetic resonance.) Analysis of x-ray diffraction patterns from protein crystals produces an electron density map, into which an atomic model of the protein is fitted. Major errors sometimes occur when fitting models in to low-resolution electron density maps (see Quality assessment for molecular models). The value of Free R is the best clue as to whether major errors may be present in a published model.
Obtaining diffraction-quality crystals of proteins remains very difficult, despite many recent advances. For every new protein sequence targeted for X-ray crystallography, about one in twenty is solved. Efforts are underway to improve this success rate.
|Crystals of the flagellar hook protein FlgE from C. jejuni produced in the Samatey lab.|
- Methods for Determining Atomic Structures
- Electron density maps
- X-ray Crystallography at Wikipedia
- Protein Crystal Gallery
- Crystal contacts
- Biological Unit
- Quality assessment for molecular models
- R value
- Free R
- Hydrogen in macromolecular models
- Water in macromolecular models
DNA X-Ray Diffraction
- Links to explanations of Rosalind Franklin's original DNA diffraction pattern, used by Watson and Crick, will be found in the See Also section of the article on DNA.
- Crystallography Made Crystal Clear: a guide for users of macromolecular models, a book by Gale Rhodes.
Notes & References
- ↑ Success Rates in Protein Crystallography
- ↑ Structural Genomics Progress Chart
- ↑ Boutet S, Lomb L, Williams GJ, Barends TR, Aquila A, Doak RB, Weierstall U, Deponte DP, Steinbrener J, Shoeman RL, Messerschmidt M, Barty A, White TA, Kassemeyer S, Kirian RA, Seibert MM, Montanez PA, Kenney C, Herbst R, Hart P, Pines J, Haller G, Gruner SM, Philipp HT, Tate MW, Hromalik M, Koerner LJ, van Bakel N, Morse J, Ghonsalves W, Arnlund D, Bogan MJ, Caleman C, Fromme R, Hampton CY, Hunter MS, Johansson L, Katona G, Kupitz C, Liang M, Martin AV, Nass K, Redecke L, Stellato F, Timneanu N, Wang D, Zatsepin NA, Schafer D, Defever J, Neutze R, Fromme P, Spence JC, Chapman HN, Schlichting I. High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography. Science. 2012 May 31. PMID:22653729 doi:10.1126/science.1217737