3jr8
Crystal Structure of BthTX-II (Asp49-PLA2 from Bothrops jararacussu snake venom) with calcium ions
Structural highlights
FunctionPA2B2_BOTJR Snake venom phospholipase A2 (PLA2) that shows a moderate enzymatic activity. It induces indirect hemolytic, anticoagulant, and cytotoxic activities. In vivo, it induces muscle necrosis, accompanied by polymorphonuclear cell infiltration, and edema in the mouse paw. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.[1] [2] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedPhospholipases A(2) (PLA(2)s) are enzymes responsible for membrane disruption through Ca(2+) -dependent hydrolysis of phospholipids. Lys49-PLA(2)s are well-characterized homologue PLA(2)s that do not show catalytic activity but can exert a pronounced local myotoxic effect. These homologue PLA(2)s were first believed to present residual catalytic activity but experiments with a recombinant toxin show they are incapable of catalysis. Herein, we present a new homologue Asp49-PLA(2) (BthTX-II) that is also able to exert muscle damage. This toxin was isolated in 1992 and characterized as presenting very low catalytic activity. Interestingly, this myotoxic homologue Asp49-PLA(2) conserves all the residues responsible for Ca(2+) coordination and of the catalytic network, features thought to be fundamental for PLA(2) enzymatic activity. Previous crystallographic studies of apo BthTX-II suggested this toxin could be catalytically inactive since a distortion in the calcium binding loop was observed. In this article, we show BthTX-II is not catalytic based on an in vitro cell viability assay and time-lapse experiments on C2C12 myotube cell cultures, X-ray crystallography and phylogenetic studies. Cell culture experiments show that BthTX-II is devoid of catalytic activity, as already observed for Lys49-PLA(2)s. Crystallographic studies of the complex BthTX-II/Ca(2+) show that the distortion of the calcium binding loop is still present and impairs ion coordination even though Ca(2+) are found interacting with other regions of the protein. Phylogenetic studies demonstrate that BthTX-II is more phylogenetically related to Lys49-PLA(2)s than to other Asp49-PLA(2)s, thus allowing Crotalinae subfamily PLA(2)s to be classified into two main branches: a catalytic and a myotoxic one. Structural, functional, and bioinformatics studies reveal a new snake venom homologue phospholipase A(2) class.,dos Santos JI, Cintra-Francischinelli M, Borges RJ, Fernandes CA, Pizzo P, Cintra AC, Braz AS, Soares AM, Fontes MR Proteins. 2011 Jan;79(1):61-78. doi: 10.1002/prot.22858. Epub 2010 Sep 27. PMID:20878713[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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