3mmp

Structure of the Qb replicase, an RNA-dependent RNA polymerase consisting of viral and host proteins

spinqualitylabelsall models 3mmp, resolution 2.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3mmp is a 4 chain structure with sequence from Escherichia coli K-12 and Escherichia virus Qbeta. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.5Å Ligands: PXN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RDRP_BPQBE This enzyme is part of the viral RNA-dependent RNA polymerase complex. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed The RNA-dependent RNA polymerase core complex formed upon infection of Escherichia coli by the bacteriophage Qbeta is composed of the viral catalytic beta-subunit as well as the host translation elongation factors EF-Tu and EF-Ts, which are required for initiation of RNA replication. We have determined the crystal structure of the complex between the beta-subunit and the two host proteins to 2.5-A resolution. Whereas the basic catalytic machinery in the viral subunit appears similar to other RNA-dependent RNA polymerases, a unique C-terminal region of the beta-subunit engages in extensive interactions with EF-Tu and may contribute to the separation of the transient duplex formed between the template and the nascent product to allow exponential amplification of the phage genome. The evolution of resistance by the host appears to be impaired because of the interactions of the beta-subunit with parts of EF-Tu essential in recognition of aminoacyl-tRNA. Structure of the Qbeta replicase, an RNA-dependent RNA polymerase consisting of viral and host proteins.,Kidmose RT, Vasiliev NN, Chetverin AB, Andersen GR, Knudsen CR Proc Natl Acad Sci U S A. 2010 Jun 15;107(24):10884-9. Epub 2010 Jun 1. PMID:20534494 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Elongation factor 3D structures RNA polymerase 3D structures

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