3o5z

Crystal structure of the SH3 domain from p85beta subunit of phosphoinositide 3-kinase (PI3K)

spinqualitylabelsall models 3o5z, resolution 2.01Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3o5z is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.01Å Ligands: CL, MPD Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function P85B_HUMAN Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Publication Abstract from PubMed Src-homology 3 (SH3) domains are involved in extensive protein-protein interactions and constitute key elements of intracellular signal transduction. Three-dimensional structures have been reported for SH3 domains of various proteins, including the 85 kDa regulatory subunit (p85) of phosphoinositide 3-kinase. However, all of the latter structures are of p85 isoform alpha and no crystal structure of the SH3 domain of the equally important isoform beta has been reported to date. In this structural communication, the recombinant production, crystallization and X-ray structure determination at 2.0 A resolution of the SH3 domain of human p85beta is described. The structure reveals a compact beta-barrel fold very similar to that of p85alpha. However, binding studies with two classes of proline-rich ligand peptides demonstrate that the ligand-binding specificity differs slightly between the SH3 domains of human p85beta and p85alpha, despite their high structural similarity. X-ray structure of the SH3 domain of the phosphoinositide 3-kinase p85beta subunit.,Chen S, Xiao Y, Ponnusamy R, Tan J, Lei J, Hilgenfeld R Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Nov 1;67(Pt 11):1328-33., Epub 2011 Oct 25. PMID:22102226 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Phosphoinositide 3-kinase 3D structures

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