3zs7

Crystal structure of pyridoxal kinase from Trypanosoma brucei

spinqualitylabelsall models 3zs7, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3zs7 is a 1 chain structure with sequence from Trypanosoma brucei brucei TREU927. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2Å Ligands: ATP, PO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function O15927_9TRYP Publication Abstract from PubMed Pyridoxal-5'-phosphate (vitamin B(6) ) is an essential cofactor for many important enzymatic reactions such as transamination and decarboxylation. African trypanosomes are unable to synthesise vitamin B(6) de novo and rely on uptake of B(6) vitamers such as pyridoxal and pyridoxamine from their hosts, which are subsequently phosphorylated by pyridoxal kinase (PdxK). A conditional null mutant of PdxK was generated in Trypanosoma brucei bloodstream forms showing that this enzyme is essential for growth of the parasite in vitro and for infectivity in mice. Activity of recombinant T. brucei PdxK was comparable to previously published work having a specific activity of 327 +/- 13 mU mg(-1) and a K(m) (app) with respect to pyridoxal of 29.6 +/- 3.9 microM. A coupled assay was developed demonstrating that the enzyme has equivalent catalytic efficiency with pyridoxal, pyridoxamine and pyridoxine, and that ginkgotoxin is an effective pseudo substrate. A high resolution structure of PdxK in complex with ATP revealed important structural differences with the human enzyme. These findings suggest that pyridoxal kinase is an essential and druggable target that could lead to much needed alternative treatments for this devastating disease. Chemical, genetic and structural assessment of pyridoxal kinase as a drug target in the African trypanosome.,Jones DC, Alphey MS, Wyllie S, Fairlamb AH Mol Microbiol. 2012 Oct;86(1):51-64. doi: 10.1111/j.1365-2958.2012.08189.x. Epub , 2012 Aug 16. PMID:22857512 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Pyridoxal kinase

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