4jno

Crystal structure of Plasmodium falciparum Erythrocyte Binding Antigen 140 (PfEBA-140/BAEBL) Region II in complex with sialyllactose

spinqualitylabelsall models 4jno, resolution 3.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4jno is a 1 chain structure with sequence from Plasmodium falciparum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3Å Ligands: SIA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q8WS31_PLAFA Publication Abstract from PubMed Plasmodium falciparum erythrocyte invasion is dependent on high-affinity recognition of sialic acid on cell-surface receptors. The Erythrocyte Binding-like (EBL) family of invasion ligands mediates recognition of sialic acid on erythrocyte glycoproteins. Erythrocyte Binding Antigen-140 (PfEBA-140/BAEBL) is a critical EBL ligand that binds sialic acid on its receptor Glycophorin C. We present here the crystal structure of the two domain receptor-binding region of PfEBA-140 in complex with a glycan containing sialic acid. The structure identifies two glycan-binding pockets unique to PfEBA-140 and not shared by other EBL ligands. Specific molecular interactions that enable receptor engagement are identified and reveal that the glycan-binding mode is distinct from that of Apicomplexan and viral cell surface recognition ligands as well as host immune factors that bind sialic acid. Erythrocyte binding experiments elucidated essential glycan contact residues and identified divergent functional roles for each receptor binding site. One of four polymorphisms proposed to affect receptor-binding was localized to a glycan binding site, providing a structural basis for altered erythrocyte engagement. The studies described here provide the first full description of sialic acid-dependent molecular interactions at the P. falciparum erythrocyte invasion interface and define a framework for development of PfEBA-140 based therapeutics, vaccines, and diagnostics assessing vaccine efficacy and natural immunity to infection. Molecular basis for sialic acid-dependent receptor recognition by Plasmodium falciparum erythrocyte binding antigen 140/BAEBL.,Malpede BM, Lin DH, Tolia NH J Biol Chem. 2013 Mar 18. PMID:23508963 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Erythrocyte binding antigen

Proteopedia Page Contributors and Editors (what is this?)

OCA