4usx

The Structure of the C-terminal YadA-like domain of BPSL2063 from Burkholderia pseudomallei

spinqualitylabelsall models 4usx, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4usx is a 3 chain structure with sequence from Burkholderia pseudomallei K96243. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.8Å Ligands: MG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q63TA4_BURPS Publication Abstract from PubMed The 1.8 A resolution crystal structure of a conserved domain of the potential Burkholderia pseudomallei antigen and trimeric autotransporter BPSL2063 is presented as a structural vaccinology target for melioidosis vaccine development. Since BPSL2063 (1090 amino acids) hosts only one conserved domain, and the expression/purification of the full-length protein proved to be problematic, a domain-filtering library was generated using beta-lactamase as a reporter gene to select further BPSL2063 domains. As a result, two domains (D1 and D2) were identified and produced in soluble form in Escherichia coli. Furthermore, as a general tool, a genomic open reading frame-filtering library from the B. pseudomallei genome was also constructed to facilitate the selection of domain boundaries from the entire ORFeome. Such an approach allowed the selection of three potential protein antigens that were also produced in soluble form. The results imply the further development of ORF-filtering methods as a tool in protein-based research to improve the selection and production of soluble proteins or domains for downstream applications such as X-ray crystallography. Selecting soluble/foldable protein domains through single-gene or genomic ORF filtering: structure of the head domain of Burkholderia pseudomallei antigen BPSL2063.,Gourlay LJ, Peano C, Deantonio C, Perletti L, Pietrelli A, Villa R, Matterazzo E, Lassaux P, Santoro C, Puccio S, Sblattero D, Bolognesi M Acta Crystallogr D Biol Crystallogr. 2015 Nov 1;71(Pt 11):2227-35. doi:, 10.1107/S1399004715015680. Epub 2015 Oct 31. PMID:26527140 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Adhesin 3D structures

Proteopedia Page Contributors and Editors (what is this?)

OCA