5fj6

Structure of the P2 polymerase inside in vitro assembled bacteriophage phi6 polymerase complex

Loading... Status: waiting for Mol* initialization Structural highlights 5fj6 is a 1 chain structure with sequence from Pseudomonas virus phi6. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 7.9Å Ligands: MN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RDRP_BPPH6 Publication Abstract from PubMed Electron cryomicroscopy can yield near-atomic resolution structures of highly ordered macromolecular complexes. Often however some subunits bind in a flexible manner, have different symmetry from the rest of the complex, or are present in sub-stoichiometric amounts, limiting the attainable resolution. Here we report a general method for the localized three-dimensional reconstruction of such subunits. After determining the particle orientations, local areas corresponding to the subunits can be extracted and treated as single particles. We demonstrate the method using three examples including a flexible assembly and complexes harbouring subunits with either partial occupancy or mismatched symmetry. Most notably, the method allows accurate fitting of the monomeric RNA-dependent RNA polymerase bound at the threefold axis of symmetry inside a viral capsid, revealing for the first time its exact orientation and interactions with the capsid proteins. Localized reconstruction is expected to provide novel biological insights in a range of challenging biological systems. Localized reconstruction of subunits from electron cryomicroscopy images of macromolecular complexes.,Ilca SL, Kotecha A, Sun X, Poranen MM, Stuart DI, Huiskonen JT Nat Commun. 2015 Nov 4;6:8843. doi: 10.1038/ncomms9843. PMID:26534841 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also RNA polymerase 3D structures

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