5lgx

Structure of Oxidised Pentaerythritol Tetranitrate Reductase

spinqualitylabelsall models 5lgx, resolution 1.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5lgx is a 1 chain structure with sequence from Enterobacter cloacae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.5Å Ligands: FMN, IPA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function P71278_ENTCL Publication Abstract from PubMed X-ray protein crystallography has, through the determination of the three-dimensional structures of enzymes and their complexes, been essential to the understanding of biological chemistry. However, as X-rays are scattered by electrons, the technique has difficulty locating the presence and position of H atoms (and cannot locate H+ ions), knowledge of which is often crucially important for the understanding of enzyme mechanism. Furthermore, X-ray irradiation, through photoelectronic effects, will perturb the redox state in the crystal. By using single-crystal spectrophotometry, reactions taking place in the crystal can be monitored, either to trap intermediates or follow photoreduction during X-ray data collection. By using neutron crystallography, the positions of H atoms can be located, as it is the nuclei rather than the electrons that scatter neutrons, and the scattering length is not determined by the atomic number. Combining the two techniques allows much greater insight into both reaction mechanism and X-ray-induced photoreduction. Combining X-ray and neutron crystallography with spectroscopy.,Kwon H, Smith O, Raven EL, Moody PC Acta Crystallogr D Struct Biol. 2017 Feb 1;73(Pt 2):141-147. doi:, 10.1107/S2059798316016314. Epub 2017 Feb 1. PMID:28177310 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Pentaerythritol tetranitrate reductase

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