5mcx

The structure of the mature HIV-1 CA hexamer in intact virus particles

Loading... Status: waiting for Mol* initialization Structural highlights 5mcx is a 24 chain structure with sequence from Human immunodeficiency virus 1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 6.8Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function B6DRA0_9HIV1 Publication Abstract from PubMed HIV-1 contains a cone-shaped capsid encasing the viral genome. This capsid is thought to follow fullerene geometry-a curved hexameric lattice of the capsid protein, CA, closed by incorporating 12 CA pentamers. Current models for core structure are based on crystallography of hexameric and cross-linked pentameric CA, electron microscopy of tubular CA arrays, and simulations. Here, we report subnanometer-resolution cryo-electron tomography structures of hexameric and pentameric CA within intact HIV-1 particles. Whereas the hexamer structure is compatible with crystallography studies, the pentamer forms using different interfaces. Determining multiple structures revealed how CA flexes to form the variably curved core shell. We show that HIV-1 CA assembles both aberrant and perfect fullerene cones, supporting models in which conical cores assemble de novo after maturation. The structure and flexibility of conical HIV-1 capsids determined within intact virions.,Mattei S, Glass B, Hagen WJ, Krausslich HG, Briggs JA Science. 2016 Dec 16;354(6318):1434-1437. PMID:27980210 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Gag polyprotein 3D structures Virus coat proteins 3D structures

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