5ui8 | pdb_00005ui8

structure of sigmaN-holoenzyme

spinqualitylabelsall models 5ui8, resolution 3.76Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5ui8 is a 6 chain structure with sequence from Escherichia coli O157:H7, Escherichia coli S88 and Klebsiella pneumoniae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.76Å Ligands: MG, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RPOA_ECOLI DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. This subunit plays an important role in subunit assembly since its dimerization is the first step in the sequential assembly of subunits to form the holoenzyme.[HAMAP-Rule:MF_00059] Publication Abstract from PubMed The bacterial sigma factors confer promoter specificity to the RNA polymerase (RNAP). One alternative sigma factor, sigmaN, is unique in its structure and functional mechanism, forming transcriptionally inactive promoter complexes that require activation by specialized AAA+ ATPases. We report a 3.4-A resolution X-ray crystal structure of a sigmaN fragment in complex with its cognate promoter DNA, revealing the molecular details of promoter recognition by sigmaN The structure allowed us to build and refine an improved sigmaN-holoenzyme model based on previously published 3.8-A resolution X-ray data. The improved sigmaN-holoenzyme model reveals a conserved interdomain interface within sigmaN that, when disrupted by mutations, leads to transcription activity without activator intervention (so-called bypass mutants). Thus, the structure and stability of this interdomain interface are crucial for the role of sigmaN in blocking transcription activity and in maintaining the activator sensitivity of sigmaN. Crystal structure of Aquifex aeolicus sigmaN bound to promoter DNA and the structure of sigmaN-holoenzyme.,Campbell EA, Kamath S, Rajashankar KR, Wu M, Darst SA Proc Natl Acad Sci U S A. 2017 Feb 21. pii: 201619464. doi:, 10.1073/pnas.1619464114. PMID:28223493 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also RNA polymerase 3D structures Sigma factor 3D structures

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