5zx3

Mycobacterium tuberculosis RNA polymerase holoenzyme with ECF sigma factor sigma H

spinqualitylabelsall models 5zx3, resolution 2.75Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5zx3 is a 6 chain structure with sequence from Mycobacterium tuberculosis H37Rv. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.751Å Ligands: ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RPOA_MYCTU DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.[HAMAP-Rule:MF_00059] Publication Abstract from PubMed Bacterial RNA polymerase employs extra-cytoplasmic function (ECF) sigma factors to regulate context-specific gene expression programs. Despite being the most abundant and divergent sigma factor class, the structural basis of ECF sigma factor-mediated transcription initiation remains unknown. Here, we determine a crystal structure of Mycobacterium tuberculosis (Mtb) RNAP holoenzyme comprising an RNAP core enzyme and the ECF sigma factor sigma(H) (sigma(H)-RNAP) at 2.7 A, and solve another crystal structure of a transcription initiation complex of Mtb sigma(H)-RNAP (sigma(H)-RPo) comprising promoter DNA and an RNA primer at 2.8 A. The two structures together reveal the interactions between sigma(H) and RNAP that are essential for sigma(H)-RNAP holoenzyme assembly as well as the interactions between sigma(H)-RNAP and promoter DNA responsible for stringent promoter recognition and for promoter unwinding. Our study establishes that ECF sigma factors and primary sigma factors employ distinct mechanisms for promoter recognition and for promoter unwinding. Structural basis for transcription initiation by bacterial ECF sigma factors.,Li L, Fang C, Zhuang N, Wang T, Zhang Y Nat Commun. 2019 Mar 11;10(1):1153. doi: 10.1038/s41467-019-09096-y. PMID:30858373 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also RNA polymerase 3D structures Sigma factor 3D structures

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