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The Transcriptional Regulator PrfA-L140H mutant from Listeria Monocytogenes in complex with a 30-bp operator PrfA-box motif
Structural highlights
FunctionPRFA_LISMO Positively regulates expression of listeriolysin, of 1-phosphadidylinositol phosphodiesterase (PI-PLC) and other virulence factors. Publication Abstract from PubMedListeria monocytogenes is a Gram-positive pathogen able to cause severe human infections. Its major virulence regulator is the transcriptional activator PrfA, a member of the Crp/Fnr family of transcriptional regulators. To establish a successful L. monocytogenes infection, the PrfA protein needs to be in an active conformation, either by binding the cognate inducer glutathione (GSH) or by possessing amino acid substitutions rendering the protein constitutively active (PrfA*). By a yet unknown mechanism, PTS-sugars repress the activity of PrfA. We therefore undertook a transposon-based approach to identify the mechanism by which PTS-sugars repress PrfA activity. For this, we screened a transposon mutant bank to identify clones able to grow in presence of glucose-6-phosphate as a sole carbon source. Surprisingly, most of the isolated transposon mutants also carried amino acid substitutions in PrfA. In transposon-free strains, the PrfA amino-acid substitution mutants displayed growth, virulence factor expression, infectivity and DNA binding, agreeing with previously identified PrfA* mutants. Hence, the initial growth phenotype observed in the isolated clone was due to the amino acid substitution in PrfA and unrelated to the loci inactivated by the transposon mutant. Finally, we provide structural evidence for the existence of an intermediate-activated PrfA state, which gives new insights into PrfA protein activation.IMPORTANCE The Gram-positive bacterium Listeria monocytogenes is a human pathogen mainly affecting elderly, immunocompromised people and pregnant women. It can lead to meningoencephalitis, septicaemia and abortion. The major virulence regulator in L. monocytogenes is the PrfA protein, a transcriptional activator. Using a growth-based selection strategy, we identified mutations in the PrfA protein leading to constitutively active virulence factor expression. We provide structural evidence for the existence of an intermediate-activated PrfA state, which gives new insights into PrfA protein activation. A novel growth based selection strategy identifies new constitutively active variants of the major virulence regulator PrfA in Listeria monocytogenes.,Hansen S, Hall M, Grundstrom C, Brannstrom K, Sauer-Eriksson AE, Johansson J J Bacteriol. 2020 Mar 16. pii: JB.00115-20. doi: 10.1128/JB.00115-20. PMID:32179627[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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