6r2n
Crystal structure of KlGlk1 glucokinase from Kluyveromyces lactis
Structural highlights
FunctionHXKG_KLULA Glukokinase specific for aldohexoses. Phosphorylates glucose and mannose, but not fructose.[1] Publication Abstract from PubMedGlucose phosphorylating enzymes are crucial in the regulation of basic cellular processes, including metabolism and gene expression. Glucokinases and hexokinases provide a pool of phosphorylated glucose in an adenosine diphosphate (ADP)- and ATP-dependent manner to shape the cell metabolism. The glucose processing enzymes from Kluyveromyces lactis are poorly characterized despite the emerging contribution of this yeast strain to industrial and laboratory scale biotechnology. The first reports on K. lactis glucokinase (KlGlk1) positioned the enzyme as an essential component required for glucose signaling. Nevertheless, no biochemical and structural information was available until now. Here, we present the first crystal structure of KlGlk1 together with biochemical characterization, including substrate specificity and enzyme kinetics. Additionally, comparative analysis of the presented structure and the prior structures of lactis hexokinase (KlHxk1) demonstrates the potential transitions between open and closed enzyme conformations upon ligand binding. Crystal Structure of Kluyveromyces lactis Glucokinase (KlGlk1).,Zak KM, Kalinska M, Wator E, Kuska K, Krutyholowa R, Dubin G, Popowicz GM, Grudnik P Int J Mol Sci. 2019 Sep 28;20(19). pii: ijms20194821. doi: 10.3390/ijms20194821. PMID:31569356[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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