7c9o

Crystal structure of DNA-bound CCT/NF-YB/YC complex (HD1CCT/GHD8/OsNF-YC2)

spinqualitylabelsall models 7c9o, resolution 2.55Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7c9o is a 5 chain structure with sequence from Oryza sativa and Oryza sativa Japonica Group. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.55Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function HD1_ORYSJ Probable transcription factor involved in the regulation of flower development. Required for the promotion of flowering under short day (SD) conditions and the suppression of flowering under long day (LD) conditions. Regulates positively the floral activator HEADING DATE 3a (HD3A) under SD and negatively under LD conditions. Publication Abstract from PubMed CCT (CONSTANS, CONSTANS-LIKE and TOC1) domain-containing proteins are a large family unique to plants. They transcriptionally regulate the processes related to photoperiodic flowering, circadian rhythms, vernalization, and other related functions. CO (CONSTANS) and HD1 (HEADING DATE 1), through the CCT domain, coordinate with the NF-YB/YC dimer to specifically target a conserved 'CCACA' motif within the downstream promotor. However, the mechanism underlying the DNA recognition by CCT domain remains unclear. Here we determined the crystal structures of the rice NF-YB/YC dimer and the florigen Hd3a-bound HD1CCT/NF-YB/YC trimer with resolutions of 2.0 A and 2.55 A, respectively. The CCT domain of HD1 displays an elongated structure containing two (alpha)-helices and two loops, tethering the Hd3a to the NF-YB/YC dimer. Helix (alpha)2 and loop 2 are anchored into the minor groove of the 'CCACA' motif, which determines the specific base recognition. Our structures reveal the interaction mechanism among the CCT domain, NF-YB/YC dimer, and the target DNA. These results not only provide new insights into the network between the CCT proteins and NF-Y subunits, but also offer references for improving productivity and global adaptability of crops by manipulating florigen expression. Structural Insight into DNA Recognition by CCT/NF-YB/YC Complexes in Plant Photoperiodic Flowering.,Shen C, Liu H, Guan Z, Junjie Y, Ting Z, Yan W, Wu C, Zhang Q, Yin P, Xing Y Plant Cell. 2020 Aug 25. pii: tpc.20.00067. doi: 10.1105/tpc.20.00067. PMID:32843433 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Nuclear transcription factor Y 3D structures of constans

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