7e3g

Crystal structure of Trypanosoma brucei cathepsin B R91C/T223C mutant in the living cell

spinqualitylabelsall models 7e3g, resolution 3.86Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7e3g is a 1 chain structure with sequence from Trypanosoma brucei brucei TREU927. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.86Å Ligands: BMA, NAG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function D6XHE1_TRYB2 Publication Abstract from PubMed Protein assemblies can be designed for development of nano-bio materials. This has been achieved by modulating protein-protein interactions. However, fabrication of highly ordered protein assemblies remains challenging. Protein crystals, which have highly ordered arrangements of protein molecules, provide useful source matrices for synthesizing artificial protein assemblies. Here, we describe construction of a supramolecular filament structure by engineering covalent and non-covalent interactions in a protein crystal. Performing in-cell crystallization of Trypanosoma brucei cysteine protease cathepsin B (TbCatB), we achieved a precise arrangement of protein molecules while suppressing random aggregation due to disulfide bonds. We succeeded in synthesizing bundled filament from the crystals by autoxidation of cysteinyl thiols after the isolation of the crystals from living cells. Design of an In-Cell Protein Crystal for the Environmentally Responsive Construction of a Supramolecular Filament.,Abe S, Pham TT, Negishi H, Yamashita K, Hirata K, Ueno T Angew Chem Int Ed Engl. 2021 May 25;60(22):12341-12345. doi:, 10.1002/anie.202102039. Epub 2021 Apr 23. PMID:33759310 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Cathepsin 3D structures

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