7vet

Crystal structure of bacterial chemotaxis-dependent pectin-binding protein SPH1118 in a closed conformation

spinqualitylabelsall models 7vet, resolution 2.25Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7vet is a 2 chain structure with sequence from Sphingomonas sp. A1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.25Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Gram-negative Sphingomonas sp. strain A1 exhibits positive chemotaxis toward acidic polysaccharide pectin. SPH1118 has been identified as a pectin-binding protein involved in both pectin chemotaxis and assimilation. Here we show tertiary structures of SPH1118 with six different conformations as determined by X-ray crystallography. SPH1118 consisted of two domains with a large cleft between the domains and substrates bound to positively charged and aromatic residues in the cleft through hydrogen bond and stacking interactions. Substrate-free SPH1118 adopted three different conformations in the open form. On the other hand, the two domains were closed in substrate-bound form and the domain closure ratio was changed in response to the substrate size, suggesting that the conformational change upon binding to the substrate triggered the expression of pectin chemotaxis and assimilation. This study first clarified that the solute-binding protein with dual functions recognized the substrate through flexible conformational changes in response to the substrate size. Substrate size-dependent conformational changes of bacterial pectin-binding protein crucial for chemotaxis and assimilation.,Anamizu K, Takase R, Hio M, Watanabe D, Mikami B, Hashimoto W Sci Rep. 2022 Jul 25;12(1):12653. doi: 10.1038/s41598-022-16540-5. PMID:35879323 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Hydroxysteroid dehydrogenase 3D structures

Proteopedia Page Contributors and Editors (what is this?)

OCA