8igu

Hexameric Ring Complex of Engineered V1-ATPase: A3(De)3_empty

spinqualitylabelsall models 8igu, resolution 2.77Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8igu is a 6 chain structure with sequence from Enterococcus hirae ATCC 9790. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.77Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function NTPA_ENTHA Involved in ATP-driven sodium extrusion. Publication Abstract from PubMed Allostery produces concerted functions of protein complexes by orchestrating the cooperative work between the constituent subunits. Here we describe an approach to create artificial allosteric sites in protein complexes. Certain protein complexes contain subunits with pseudo-active sites, which are believed to have lost functions during evolution. Our hypothesis is that allosteric sites in such protein complexes can be created by restoring the lost functions of pseudo-active sites. We used computational design to restore the lost ATP-binding ability of the pseudo-active site in the B subunit of a rotary molecular motor, V(1)-ATPase. Single-molecule experiments with X-ray crystallography analyses revealed that binding of ATP to the designed allosteric site boosts this V(1)'s activity compared with the wild-type, and the rotation rate can be tuned by modulating ATP's binding affinity. Pseudo-active sites are widespread in nature, and our approach shows promise as a means of programming allosteric control over concerted functions of protein complexes. Design of allosteric sites into rotary motor V(1)-ATPase by restoring lost function of pseudo-active sites.,Kosugi T, Iida T, Tanabe M, Iino R, Koga N Nat Chem. 2023 Nov;15(11):1591-1598. doi: 10.1038/s41557-023-01256-4. Epub 2023 , Jul 6. PMID:37414880 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also ATPase 3D structures

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