N-acetylneuraminate lyase

spinqualitylabelsall models N-acetylneuraminate lyase dimer with modified Lys-pyruvate complex with ethylene glycol and triethylene glycol (PDB ID 5zka) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Function N-acetylneuraminate lyase or N-acetylneuraminic acid aldolase or D-sialic acid aldolase (NANL) is a class I aldolase which reversibly catalyses the cleavage of N-acetylneuraminic acid (sialic acid) to N-acetylmannosamine and pyruvate. Relevance NANL catalyses the rate-limiting step of two biocatalytic reactions producing sialic acid in industry. Structural highlights NANL, an aldolase class I enzyme tetramer, is characterized by TIM-barrel fold and reaction mechanism which involves A Schiff base intermediate formed by covalently bond between a conserved Lys side chain and pyruvate. The Schiff base forms H-bond interactions with Ser, Thr, and conserved Tyr residue also via a water molecule (shown as red sphere). 3D structures of N-acetylneuraminate lyase N-acetylneuraminate lyase 3D structures

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