Pre-mRNA-splicing factor

spinqualitylabelsall models Yeast pre-mRNA-splicing factor 8 (green) complex with AAR2 (cyan) (PDB entry 3zef) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Function Pre-mRNA-splicing factors (Prp) are the proteins which associate with mRNA at its various splicing sites to produce alternative splicing. Prp3b is part of the U2 small nuclear ribonucleoproteins complex (U2 snRNP) Prp8 functions as a scaffold which mediates the ordered assembly of spliceosomal proteins and snRNAs. For details see Prp8 Prp18 and Bud31 are essential for the catalytic step in pre-mRNA splicing process. snRNA-associated-splicing factor 24 binds preferentially to the U4/U6 hybrid snRNAs. For details see Prp24 For Prp19 see Prp19 Prp31 functions in forming the U4/U5-U6 tri-snRNP. For Prp40 see Prp40. Prpcwc2 is a yeast splicing factor which plays a role in the generation of a functional splicing center Prp RBM22 is the human homolog of Prpcwc2. Prpcwc26 or Bud13 belongs to the pre-mRNA retention and splicing complex RES. Disease Mutations in Prp8 and Prp31 cause retinitis pigmentosa. Structural highlights Prp8 is a multi-domain protein containing an RNA-binding domain and a Jab1/MPN domain.

3D Structures of pre-mRNA splicing factors

Updated on 26-August-2018

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• Pre-mRNA-splicing factor 2
  • Prp8 – hPrp P33 subunit + Ser/Thr-protein kinase – human
    
  • Prp24 – hPrp P33 subunit
• Pre-mRNA-splicing factor 3
  • Prp19 – hPrp PWI domain - NMR
    
  • Prp40, 4yhv – yPrp C-terminal - yeast
    
  • 4yhw – yPrp C-terminal + snRNA
    
• Pre-mRNA-splicing factor 3B
  • 3lqv, 2f9d, 2f9j – hPrp residues 377-415 + pre-mRNA branch site protein 14
    
  • 2fho - hPrp residues 379-424 + spliceosomal protein P14 - NMR
    
  • 2do5 – hPrp subunit 2 – NMR
    
  • 1x5t - hPrp subunit 4 – NMR
    
  • 5gvq - hPrp subunit 4 RNA-binding domain – NMR
    
  • 6en4 - hPrp subunits 1,3,5 + SF3B14B
    
  • 5ife - hPrp subunits 1,3,5 + PHD finger-like protein 5A
    
  • 5zya - hPrp subunits 1,3,5 + PHD finger-like protein 5A + splicing modulator
    
• Pre-mRNA-splicing factor 8
  • 3sbt, 4ilh – yPrp RNAseH like domain + A1 cistron-splicing factor AAR2
    
  • 4jk7, 4jk9, 3e9o, 3e9p, 3e66 - yPrp RNA-binding domain
    
  • 4ilj, 4jk8, 4jka, 4jkb, 4jkc, 4jkd, 4jke, 4jkf, 4jkg, 4jkh - yPrp RNA-binding domain (mutant)
    
  • 3zef, 4i43 – yPrp + A1 cistron-splicing factor AAR2
    
  • 2og4, 3sbg - yPrp Jab1/MPN domain
    
  • 4bgd, 5dca, 5m52, 5m5p – yPrp Jab1/MPN domain + pre-mRNA-splicing helicase BRR2
    
  • 4kit – yPrp Jab1/MPN domain + U5 small nuclear ribonucleoprotein helicase
    
  • 4ilg – yPrp Jab1/MPN+RNA-binding domains + A1 cistron-splicing factor AAR2
    
  • 3lru – hPrp residues 1831-1990
    
  • 3e9l, 3enb – hPrp endonuclease domain
    
  • 2p8r – CePrp C terminal (mutant) – Caenorhabditis elegans
    
  • 2p87 – CePrp C terminal
    
• Pre-mRNA-splicing factor 9
  • 4dgw – yPrp + Prp 21 + Prp 11
• Pre-mRNA-splicing factor 11
  • 4dgw – yPrp + Prp 21 + Prp 9
• Pre-mRNA-splicing factor 18
  • 2dk4 – hPrp splicing factor motif domain - NMR
    
  • 1dvk – yPrp functional domain
    
• Pre-mRNA-splicing factor 19
  • 3lrv – yPrp WD40 domain
    
  • 2bay – yPrp U-box
    
  • 1n87 - yPrp U-box - NMR
    
• Pre-mRNA-splicing factor 20
  • 2i2y – Prp RNA-binding domain + RNA – Streptococcus – NMR
    
  • 2i38 - hPrp RNA-binding domain (mutant) – NMR
    
• Pre-mRNA-splicing factor 21
  • 4dgw – yPrp + Prp 9 + Prp 11
• snRNA-associated-splicing factor 24
  • 2l9w – yPrp C terminal – NMR
    
  • 2kh9 – yPrp residues 115-197 + RNA – NMR
    
  • 2go9 – yPrp RNA-binding domains – NMR
    
  • 2ghp - yPrp RNA-binding domains
• Pre-mRNA-splicing factor 31
  • 2ozb – hPrp + RNA + tri-snRNP
    
  • 3siu, 3siv – hPrp + NHP2-like protein + snRNP
    
• Pre-mRNA-splicing factor 38A
  • 4rz9, 4rza – hPrp
    
  • 5f5s – hPrp + microfibrillar-associated protein 1
    
• Pre-mRNA-splicing factor 40
  • 1o6w – yPrp WW domain – yeast - NMR
    
  • 2b7e – yPrp FF1 domain - NMR
    
  • 2kfd – yPrp FF4 domain - NMR
    
• Pre-mRNA-splicing factor 43P
  • 2xau, 3kx2 – yPrp + ADP
• Pre-mRNA-splicing factors in spliceosome
  • 3jb9 – yPrp
    
  • 3jcm, 5gam, 5gan, 5gao, 5gap, 5gmk, 5lj3, 5lj5, 5lqw, 5mps, 5mq0, 5mqf, 5wsg, 6g90, 6exn, 6bk8, 5ylz, 5y88 – yPrp - CryoEM
    
  • 3jcr, 5o9z – hPrp - CryoEM
    
• Pre-mRNA-splicing factor cwc2
  • 3tp2, 3u1l, 3u1m – yPrp
    
• Pre-mRNA-splicing factor cwc26
  • 2mkc – yPrp + U2 SNRNP component IST3 + Pre-mRNA leakage protein
    
  • 4uqt – yPrp + U2 SNRNP component IST3
    
• Pre-mRNA-splicing factor bud31
  • 2my1 – yPrp - NMR
    
• Pre-mRNA-splicing factor sad1
  • 4msx – yPrp
    
• Pre-mRNA-splicing factor RBM22
  • 2ytc – yPrp RNA-binding domain - NMR
    
• Pre-mRNA-splicing factor RDS3
  • 2k0a – yPrp - NMR
    
• Pre-mRNA-splicing factor URN1
  • 2juc – yPrp FF domain - NMR
    

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