Laccase

spinqualitylabelsall models CotA laccase complex with glycerol, O2 and Cu+2 (orange), 1w6l Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Function Laccase (Lac) is a multi-copper protein which uses molecular oxygen to oxidize various aromatic and non-aromatic compounds by a radical-catalyzed reaction mechanism</ref>. Laccase 2 (Lac2) acts in lignin degradation and in detoxification of lignin products. Typically, laccases show a three cupredoxin-domain folding. Two-domain laccase or small lactase have unusual resistance to inhibitors. CotA laccase belongs to the multi-copper oxidase family. The multi-copper oxidases constitute a family of enzymes whose principal members are laccase (benzenediol oxygen oxidoreductase, EC 1.10.3.2), ascorbate oxidase (L-ascorbate oxygen oxidoreductase, EC 1.10.3.3) and ceruloplasmin (Fe(II) oxygen oxidoreductase, EC 1.16.3.1). Similar to the other laccases the three dimensional structure of CotA 1w6l comprises three cupredoxin domains and four copper ions organised in Two copper centers: a mononuclear blue type 1 copper center and a trinuclear center. For laccase with nitrotyrosine modification see Nitrotyrosine. Relevance Laccase from various fungi is used in adsorption of dyes from polluted environment</ref>. Laccases play an important role in food industry, paper and pulp industry, textile industry, synthetic chemistry, cosmetics, soil bioremediation and biodegradation of phenolic pollutants. Structural highlights The trinuclear center of CotA laccase has two type 3 copper ions, that can be anti-ferromagnetically coupled through an hydroxyl moiety in between them, and one type 2 copper ion.‡ The mononuclear copper is able to accept an electron from a variety of phenolic substrates and then transmit it to the trinuclear centre. 3D structures of laccase Laccase 3D structures

3D structures of laccase

Updated on 16-May-2019

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• Laccase
  • 1w6l – Lac + Cu – Rigidoporus lignosus
    
  • Nitrotyrosine – Lac + Cu – Trametes maxima
    
  • Laccase 3D structures – Lac + Cu – Trametes ochracea
    
  • 3x1b – Lac + Cu – Lentinus
    
  • 5anh – Lac + Cu – Basidiomycete
    
  • 5ehf – Lac + Cu – Antrodiella faginea
    
  • 4a2d, 4a2e, 4a2f, 4a2g, 4a2h, 5a7e – Lac + Cu – Coriolopsis gallica
    
  • 4jhu, 4jhv – Lac + Cu – Coriolopsis caperata
    
  • 3t6v, 3t6w, 3t6x, 3t6z, 3t71 – Lac + Cu – Steccherinum ochraceum
    
  • 3sqr, 4x4k – BaLac + Cu – Botrytis aclada
    
  • 3v9e – BaLac (mutant) + Cu
    
  • 2xuw, 2xvb – TtLac – Thermus thermophilus
    
  • 2yap, 5jx9, 5k0d, 5k15, 5k3k, 5k5k, 5k7a, 5k84 – TtLac + Cu
    
  • 2xu9 – TtLac (mutant) + Cu
    
  • 2yae, 5jrr – TtLac + Cu + O2
    
  • 2yaf, 2yah, 2yam, 2yao, 2yaq, 2yar, 5afa – TtLac + Cu + OH
    
  • 2xyb – Lac + Cu + O2 – Pycnoporus cinnabarinus
    
  • 3pps – Lac + Cu + O2 – Thielavia arenaria
    
  • 3cg8 – Lac + Cu + O – Streptomyces coelicolor
    
  • 3tbb – Lac + Cu + O2 – Streptomyces viridosporus
    
  • 4f7k – Lac – uncultured bacterium
    
  • 5nq7, 5nq8, 5nq9 – Lac + Cu + O2 – Trametes sanguinea
    
  • 5z1x, 5z22 – Lac + Cu + O2 – Cerrena
    
• Laccase 1
  • 3qpk – MaLac1 + Cu – Melanocarpus albomyces
    
  • 1gw0, 2ih8, 2ih9, 2q9o – MaLac1 + Cu + O
    
  • 3dkh – MaLac1 (mutant) + Cu + O2
    
  • 3fu7, 3fu8, 3fu9 – MaLac1 + Cu + O2 + phenol derivative
    
  • 1hfu – icfLac1 + Cu – inky cap fungus
    
  • 5ldu – wrfLac1 + Cu – white-rot fungus
    
  • 6f5k – Lac1 + Cu + OH – Myceliophthora thermophila
    
• Laccase 2
  • 1a65 – icfLac2 + Cu + O
    
  • 1gyc, 3fpx, 3kw7, 3pxl – wrfLac2 + Cu
    
  • 3v9c – wrfLac2 + Cu + O2
    
  • 1kya – Lac2 + Cu + arylamine – Trametes versicolor
    
  • 5e9n, 6rgh – SmLac2 + Cu – Steccherinum murashkinskyi
    
  • 5mej, 5mew, 5mhu, 5mhv, 5mhw, 5mhx, 5mhy, 5mhz, 5mi1, 5mi2, 5mia, 5mib, 5mic, 5mid, 5mie, 5mig, 6rgp, 6rhh, 6rhi, 6rho, 6rhp, 6rhr, 6rhu, 6rhx, 6ri0, 6ri2, 6ri4, 6ri6, 6ri8, 6rii, 6rik, 6ril – SmLac2 + Cu + O2
    
• Two-domain laccase
  • 2zwn – Lac + Cu + Cu-O-Cu – metagenomes
    
  • 5lhl – SgLac + Cu + O2 – Streptomyces griseoflavus
    
  • 5mkm, 5o3k, 5o4i, 5o4q, 6fc7, 6fdj – SgLac (mutant) + Cu + O2 + O
    
• CotA laccase
  • 2x87, 2x88, 1w6l, 1w6w, 1w8e, 1gsk – BsCotAl – Bacillus subtilis
    
  • 2bhf - BsCotAl reduced
    
  • 2wsd, 4ako, 4akp, 4akq – BsCotAl (mutant)
    
  • 1of0, 3zdw - BsCotAl + ABTS
    
  • 4a66, 4a67, 4a68 - BsSCP (mutant) + Cu + peroxide
    
• CotS laccase
  • 1nps – CotSl N terminal – Myxococcus xanthus
• Laccase
  • 3div - Lac - Cerrena maxima
    
• CueO laccase

See Blue copper oxidase CueO

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