3w9w: Difference between revisions
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==Crystal structure of DING protein== | |||
=== | <StructureSection load='3w9w' size='340' side='right' caption='[[3w9w]], [[Resolution|resolution]] 1.35Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3w9w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Unidentified_prokaryotic_organism Unidentified prokaryotic organism]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W9W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3W9W FirstGlance]. <br> | |||
==Disease== | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2v3q|2v3q]], [[3g62|3g62]], [[2q9t|2q9t]], [[3w9v|3w9v]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3w9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3w9w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3w9w RCSB], [http://www.ebi.ac.uk/pdbsum/3w9w PDBsum]</span></td></tr> | |||
</table> | |||
== Disease == | |||
[[http://www.uniprot.org/uniprot/PHBP_UNKP PHBP_UNKP]] May be involved in atherosclerosis. | [[http://www.uniprot.org/uniprot/PHBP_UNKP PHBP_UNKP]] May be involved in atherosclerosis. | ||
== Function == | |||
[[http://www.uniprot.org/uniprot/PHBP_UNKP PHBP_UNKP]] Phosphate-binding protein.<ref>PMID:18076037</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
After crystallization of a certain protein-RNA complex, well diffracting crystals were obtained. However, the asymmetric unit of the crystal was too small to locate any components. Mass spectrometry and X-ray crystal structure analysis showed that it was a member of the DING protein family (HPBP). Surprisingly, the structure of HPBP reported previously was also determined accidentally as a contaminant, suggesting that HPBP has a strong tendency to crystallize. Furthermore, DING proteins were reported to relate in disease. These observations suggest that DING has potential for application in a wide range of research fields. To enable further analyses, a system for preparation of HPBP was constructed. As HPBP was expressed in insoluble form in Escherichia coli, it was unfolded chemically and refolded. Finally, a very high yield preparation method was constructed, in which 43 mg of HPBP was obtained from 1 L of culture. Furthermore, to evaluate the validity of refolding, its crystal structure was determined at 1.03 A resolution. The determined structure was identical to the native structure, in which two disulfide bonds were recovered correctly and a phosphate ion was captured. Based on these results, it was concluded that the refolded HPBP recovers its structure correctly. | |||
Crystal structure analysis, overexpression and refolding behaviour of a DING protein with single mutation.,Gai Z, Nakamura A, Tanaka Y, Hirano N, Tanaka I, Yao M J Synchrotron Radiat. 2013 Nov;20(Pt 6):854-8. doi: 10.1107/S0909049513020694., Epub 2013 Sep 29. PMID:24121327<ref>PMID:24121327</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== | ==See Also== | ||
*[[Phosphate-binding protein|Phosphate-binding protein]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Unidentified prokaryotic organism]] | [[Category: Unidentified prokaryotic organism]] | ||
[[Category: Gai, Z Q | [[Category: Gai, Z Q]] | ||
[[Category: Hirano, N | [[Category: Hirano, N]] | ||
[[Category: Nakamura, A | [[Category: Nakamura, A]] | ||
[[Category: Tanaka, I | [[Category: Tanaka, I]] | ||
[[Category: Tanaka, Y | [[Category: Tanaka, Y]] | ||
[[Category: Yao, M | [[Category: Yao, M]] | ||
[[Category: Phosphate]] | [[Category: Phosphate]] | ||
[[Category: Phosphate binding protein]] | [[Category: Phosphate binding protein]] | ||
[[Category: Transport protein]] | [[Category: Transport protein]] | ||
Revision as of 07:09, 21 December 2014
Crystal structure of DING protein
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