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| oxidoreductase, EC 1.10.3.3) and ceruloplasmin (Fe(II) oxygen | | oxidoreductase, EC 1.10.3.3) and ceruloplasmin (Fe(II) oxygen |
| oxidoreductase, EC 1.16.3.1). Similar to the other laccases the three dimensional structure of CotA [[1w6l]] comprises three cupredoxin domains and four copper ions organised in <scene name='CotA_laccase/Copper_centers/5'>Two copper centers</scene>: | | oxidoreductase, EC 1.16.3.1). Similar to the other laccases the three dimensional structure of CotA [[1w6l]] comprises three cupredoxin domains and four copper ions organised in <scene name='CotA_laccase/Copper_centers/5'>Two copper centers</scene>: |
| a <scene name='CotA_laccase/Mononuclear_t1copper/1'>mononuclear blue type 1 copper center</scene> and <scene name='40/404916/Cv/2'>a trinuclear center</scene>.<ref>PMID:11514528</ref> | | a <scene name='40/404916/Cv/3'>mononuclear blue type 1 copper center</scene> and <scene name='40/404916/Cv/2'>a trinuclear center</scene>.<ref>PMID:11514528</ref> |
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| == Structural highlights == | | == Structural highlights == |
Revision as of 09:56, 21 December 2015
| Function
CotA laccase belong to the multi-copper oxidase family.
The multi-copper oxidases constitute a family of enzymes whose
principal members are laccase (benzenediol oxygen oxidoreductase,
EC 1.10.3.2), ascorbate oxidase (L-ascorbate oxygen
oxidoreductase, EC 1.10.3.3) and ceruloplasmin (Fe(II) oxygen
oxidoreductase, EC 1.16.3.1). Similar to the other laccases the three dimensional structure of CotA 1w6l comprises three cupredoxin domains and four copper ions organised in Two copper centers:
a mononuclear blue type 1 copper center and a trinuclear center.[1]
Structural highlights
The trinuclear center has two type 3 copper ions, that can be anti-ferromagnetically
coupled through an hydroxyl moiety in between them, and one
type 2 copper ion.‡ The mononuclear copper is able to accept an
electron from a variety of phenolic substrates and then transmit
it to the trinuclear centre.
- ↑ Hullo MF, Moszer I, Danchin A, Martin-Verstraete I. CotA of Bacillus subtilis is a copper-dependent laccase. J Bacteriol. 2001 Sep;183(18):5426-30. PMID:11514528
|
3D structures of CotA laccase
Updated on 21-December-2015
{"openlevels":0}
- CotA laccase
- 1w6l, 2x88, 1w6l, 1w6w, 1w8e, 1gsk – BsCotAl – Bacillus subtilis
- 2bhf - BsCotAl reduced
- 2wsd, 4ako, 4akp, 4akq – BsCotAl (mutant)
- 1of0, 3zdw - BsCotAl + EBS
- 4a66, 4a67, 4a68 - BsSCP (mutant) + Cu + peroxide
- 1uvw - BsCotAl + ABTS
- CotS laccase
- 1nps – CotSl N terminal – Myxococcus xanthus
- CueO laccase
See Blue copper oxidase CueO
References
