GTP-binding protein

spinqualitylabelsall models E. coli small GTP-binding protein ERA complex with GDP, TRIS and sulfate (PDB code 3ieu) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D File:2nzj.png Crystal Structure of GTP-binding protein Rem1 tetramer complex with nucleotide 2nzj Function GTP-binding proteins or G proteins are transmitting signals outside the cell which cause changes within the cell. They act as molecular switches which are on when binding GTP and off when binding GDP. GTP-binding proteins belong to two families: heterotrimeric G proteins and small GTPases. The structure of small GTPase is homologous to that of the α subunit of heterotrimeric G protein. See details on GTP-binding protein LepA in LepA. An activated G protein-coupled receptor (human β-2 adrenergic receptor in blue ) in a complex with a heterotrimeric G protein (3 subunits:reddish to orange-brown) and hormone (gold) (3sn6), resolution 3.2Å. The boundaries of the membrane in which the GPCR sits are represented in light green. Structural highlights E. coli small GTPase ERA binds GDP in a cavity formed by 3 highly conserved regions.

3D Structures of GTP-binding protein

Updated on 17-March-2016

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• GTP-binding protein
  • 2nzj, LepA – yGtr1 + Gtr2 – yeast
    
  • 3sn6 – hGBP TGS domain – NMR - human
    
  • 3kxk, 3kxl – SsHflx (mutant) - Sulfolobus solfataricus
    
  • 2qtf – SsHflx
    **1ega – EcERA – Escherichia coli
    
  • 2dyk – TtEngA N terminal – Thermus thermophilus - Cryo EM
    
  • 3cb4 - EcLepA – Escherichia coli
    
  • 2ywe – AaLepA – Aquifex aeolicus
    
  • 1sul – BsYsxC - Bacillus subtilis
    
  • 2dwq – TtYchF
    
  • 1udx – TtObg
    
  • 1wxq – GBP – Pyrococcus horikoshii
    
  • 1yr6 - PaGBP – Pyrococcus abyssi
    
  • 1ni3 – YchF – Schizosaccharomyces pombe
    
• GTP-binding protein complex with nucleotide
  • 2nzj – hRem1 + GDP
    
  • 3cbq – hRem2 + GDP
    
  • 3q85 – hRem2 G domain + GTP analog
    
  • 4aii - rRem2 G domain + GDP - rat
    
  • 2ohf – hOla1 + AMP-PCP
    
  • 1e0a – hGBP (mutant) + GIP
    
  • 2b8w, 2d4h – hGBP N terminal + GMP
    
  • 2b92 - hGBP N terminal + GDP
    
  • 2bc9 - hGBP N terminal + GPPNHP
    
  • 1yr7, 1yr8 – PaGBP + GTP
    
  • 1yr9, 1yra, 1yrb, 2oxr – PaGBP + GDP
    
  • 3kxi, 2qth – SsHflx + GDP
    
  • 2ywf – AaLepA + GMP-PNP
    
  • 2ywg - AaLepA + GTP
    
  • 2ywh - AaLepA + GDP
    
  • 1yzn – yPt1 + GPPNHP
    
  • 1ky2 – yPt7 + GPPNHP
    
  • 1ky3 – yPt7 + GDP
    
  • 3rwm, 3rwo – yPt32 + GPPNHP
    
  • 1ek0 – yPt51 + GPPNHP
    
  • 2hjg, 4dcs, 4dct, 4dcu, 4dcv – BsEngA + GDP
    
  • 1svi – BsYsxC + GDP
    
  • 1svw – BsYsxC + GMPPNP
    
  • 1lnz – BsObg + GTP
    
  • 2dby – TtYchF + GDP
    
  • 1wf3 – Tt1341 + GNP
    
  • 2gao - hSar1A + GDP
    
  • 2fa9 – ChSar1B (mutant) + GDP – Chinese hamster
    
  • 2fmx - ChSar1B + GDP
    
• GTP-binding protein complex with protein
  • 3deg – EcLepA + A-tRNA + P-tRNA + RPS12 + RPL11 – Cryo EM
    
  • 3cue – yPt1 + transport protein subunits
    
  • 2bcg, 1ukv - yPt1 + secretory pathway GDP-dissociation inhibitor
    
  • 3cpj – yPt31 + Rab GDP-dissociation inhibitor
    
  • 1m2o – ySar1 + protein transport protein sec23
    
  • 3syn – BsFlhf + ATP-binding protein
    
• GTP-binding protein 3 (Atlastin-1)
  • 4idn – hGDP3 cytoplasmic domain + GNP
    
  • 4ido, 4idq, 3q5d, 3q5e, 3qnu, 3qof – hGDP3 cytoplasmic domain + GDP
    
• G protein bound to a G protein-coupled receptor
  • 3sn6 - human β-2 adrenergic receptor + cameloid antibody fragment bound by guanine nucleotide-binding protein G. This is the first structure of an activated G protein-coupled receptor in a complex with its G protein.
• Ras-related small GTP-binding protein
  • 3llu – hC nucleotide-binding domain
    
• Ras-related small GTP-binding protein complex with nucleotide
  • 2l0x – rRheb + GDP
    
  • 2gjs – hRad1 + GDP
    
  • 3q72, 3q7p, 3q7q – hRad G-domain + GTP analog
    
  • 2dpx – hRad GTPase domain
    
  • 2gf0 – hDiRas1 + GDP
    
  • 2erx – hDiRas2 + Pi + GDP
    
  • 1x3s – hRab-18 + GPPNHP
    
  • 1yu9 - hRab-4A + GPPNHP
    
  • 2atx – hTc10 + GPPNHP
    
  • 1g16 – ySec4 + GDP
    
  • 1g17 - ySec4 + GIP
    
• Ras-related small GTP-binding protein complex with protein
  • 3kuc – hRap-1A + proto oncogene Ser/Thr protein kinase
    
  • 3cf6 - hRap-1A + Rap guanine nucleotide exchange factor 4
    
  • 3brw - hRap-1B + Rap GTPase-activating protein
    
  • 1z0k – hRab-4 + rabenosyn-5
    
  • 3tw8 – hRab-1C + DENND
    
  • 4ojk – hRab-11B + cGMP-dependent protein kinase 2
    
• Interferon-induced GTP-binding protein
  • 3ljb – hMx1 stalk region
    
  • 3szr – hMx1 residues 33-662
    
  • 3zys – hMx1 + dynamin-1
    
• Rho-related small GTP-binding protein
  • 2j1l – hRhoD
• Rho-related small GTP-binding protein complex with nucleotide
  • 2cls – hRho6 + GTP
    
  • 2fv8 - hRhoB + GDP
    
  • 2gcn – hRhoC + GDP
    
  • 2gco – hRhoC + GPPNHP
    
  • 1z2c - hRhoC (mutant) + GMPPNP
    
  • 2gcp – hRhoC + GSP
    
  • 1m7b - hRhoE + GTP
    
  • 1gwn - mRhoE + GTP
    
  • 1a4r - hCdc42 (mutant) + GDP
    
  • 1an0 - hCdc42 + GDP
    
  • 2qrz - hCdc42 + GCP
    
• Rho-related small GTP-binding protein complex with protein
  • 2v55 – hRhoE + Rho-associated protein kinase
    
  • 3q3j – hRho6 + plexin-A2 nucleotide-binding domain
    
  • 2rex – hRho6 + plexin-B1
    
  • 1cc0 – hRhoA + GDP-dissociation inhibitor + GDP
    
  • 3a58 – yRho1 (mutant) + exocyst complex component Sec3
    
  • 1gzs – Cdc42 + guanine nucleotide exchange factor – Salmonella typhimurium
    
  • 2kb0 - hCdc42 (mutant)
    
  • 1kz7 - hCdc42 + guanine nucleotide exchange factor
    
  • 1kzg - hCdc42 (mutant) + guanine nucleotide exchange factor (mutant)
    
  • 2dfk – hCdc42 + collybistin II
    
  • 2wm9, 2wmn, 2wmo - hCdc42 + DOCK9
    
  • 3eg5 - hCdc42 + protein diaphanous homolog 1 + GMPPNP
    
  • 3gcg - hCdc42 + MAP
    
  • 3n3v - hCdc42 + AMP-protein transferase
    
  • 1nf3 - hCdc42 + Par6
    
  • 2ase - hCdc42 (mutant) - NMR
    
  • 1ki1 – hCdc42 + intersectin long form
    
  • 1ees – hCdc42 + MapK3 peptide - NMR
    
  • 1doa - hCdc42 + GDP-dissociation inhibitor
    
  • 2ngr – hCdc42 + GTPase-activating protein C terminal + GDP
    
  • 1am4 – hCdc42 + GTPase-activating protein rhoGAP + GNP
    
  • 1grn – hCdc42 + GTPase-activating protein rhoGAP + GDP
    
  • 1cf4 – hCdc42 (mutant) + ACK GTPase-binding domain + GNP - NMR
    
  • 1cee – hCdc42 + WASP GTPase-binding domain + GCP - NMR
    
  • 2odb – hCdc42 + PAK6 CRIB domain + GCP
    
  • 3qbv – hCdc42 (mutant) + intersectin + GDP
    
  • 4did – hCdc42 + inositol phosphate phosphatase + GDP
    
  • 1cf4 – hCdc42 (mutant) + dedicator of cytokinesis protein 8
    
  • 4itr – hCdc42 + AMP-protein transferase + GDP + AMP
    
• Rac-like GTP-binding protein
  • 2wbl – AtArac2 + Rho of plant guanine nucleotide exchange factor 8 – Arabidopsis thaliana
    
  • 3bwd – AtArac6
    
  • 2nty – AtArac5 + prone8
    
  • 2j0v – AtArac7 (mutant)
    
• Rab-related small GTP-binding protein
  • 2ys8 – hRabJ DNAJ domain – NMR
    
• Flagella-associated GTP-binding protein
  • 2px0 – BsFlhF + GMP-PNP
    
  • 2px3 – BsFlhF + GTP
    
• Small GTP-binding protein
  • 2rhd – Rab-1a – Cryptosporidium parvum
    
  • 1yzq - hRab-6 + GNP
    
  • 3qq5 – GBP – Thermotoga neapolitana
    
• Small GTP-binding protein ERA
  • 1ega – EcERA
    
  • 3ieu – EcERA + GDP
    
  • 3iev – AaERA + RNA + Mg-GNP
    
  • 1x1l – TtERA + RNA – Cryo EM
    
  • 1x18 – TtERA + RNA + RPS2 + RPS7 + RPS11 + RPS18 – Cryo EM
    
• Small GTP-binding protein Rheb
  • 1xtq – hRheb + GDP
    
  • 1xts - hRheb + GTP
    
  • 1xtr - hRheb + GPPNHP
    
  • 3sea - hRheb + GDP + GNP
    
  • 4o25 – mRheb + GTP - mouse
    
  • 4o2l - mRheb (mutant) + GTP
    
  • 4o2r - mRheb (mutant) + GDP
    
  • 3t5g – hRheB + GMP-PDE δ
    
• Small GTP-binding protein Gem
  • 2cjw, 2ht6, 2g3y – hGem G domain + GDP
    
• Small GTP-binding protein Ran
  • 2mmc, 2mmg – hRan
    
• Ran binary complex
  • 3gj0 – hRan + GDP
    
  • 1i2m – hRan + regulator of chromosome condensation 1
    
  • 1byu – Ran + GDP - dog
    
  • 1qg2, 1qg4, 3ran – dRan (mutant) + GDP
    
  • 2bku – dRan + importin
    
• Ran ternary complex
  • 1rrp – hRan + Nup358 + GTP analog
    
  • 1qbk – hRan + karyopherin β2 + GTP analog
    
  • 1ibr – hRan + importin β1 subunit + GTP analog
    
  • 4ol0 – hRan + importin 12 + GTP
    
  • 3ea5 – hRan (mutant) + importin β1 subunit + GDP
    
  • 4c0q - hRan (mutant) + importin 12 + GTP
    
  • 3nc1 – hRan (mutant) + exportin 1 + GTP
    
  • 3ch5, 3gj3, 3gj4, 3gj5, 3gj6, 3gj7, 3gj8 – hRan + Nup153 + GDP
    
  • 3w3z – dRan + importin β1 subunit + GTP
    
• Ran quaternary complex
  • 3gjx – hRan + snurportin 1 + exportin 1 + GTP
    
  • 3nby, 3nbz, 3nc0 – hRan (mutant) + snurportin 1 + exportin 1 + GTP
    
  • 1k5d – hRan + Ran-specific GTPase-activating protein + Ran GTPase activating protein 1 + GTP analog
    
  • 1k5g – hRan + Ran-specific GTPase-activating protein + Ran GTPase activating protein 1 + GDP
    
  • 4gpt, 4gmx, 4hat, 4hb2, 4hb3 – hRan + Ran-specific GTPase-activating protein + exportin 1 + GNP
    
  • 4hat, 4hau, 4hav, 4haw, 4hax, 4hay, 4haz, 4hb0, 4hb4 – hRan + Ran-specific GTPase-activating protein + exportin 1 + GNP + antibiotic
    
  • 3zjy – hRan (mutant) + importin 13 + EIF-1A + GTP
    
  • 3a6p – dRan + pre-microRNA + exportin 5 + peptide
    
  • 3icq – yRan + RNA + exportin T
    
  • 3m1i – yRan + Ran-specific GTPase-activating protein + exportin 1 + GTP
    
• Small GTPase h-Ras

See GTPase-HRAS

See Also

• G protein-coupled receptor

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