Elongation factor

spinqualitylabelsall models Structure of EF-Tu (cyan and magenta) with EF-Ts (green and yellow) (PDB entry 1efu) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Elongation factors (EF) facilitate translational elongation during the formation of peptide bonds in the ribosome. EF-selB is selenocysteine-specific EF. See SelB EF-Tu or EF 1-α (elongation factor thermo unstable) is a prokaryotic EF. EF-Tu contributes to translational accuracy. It catalyzes the addition of aminoacyl tRNA. EF-Ts or EF 1-β (elongation factor thermo stable) catalyzes the release of GDP from EF-Tu. EF-G translocates the peptidyl tRNA from the A site to the P site while moving the mRNA through the ribosome. EF-SII helps RNA polymerase II to bypass blocks to elongation. EF-ELL2 enhances polyadenylation and exon skipping with the gene encoding the immunoglobulin heavy-chain complex. EF-GreA or GreB are cleavage factors allowing the resumption of elongation. EF-NusA recruits translesion DNA polymerases to gaps encountered during translation. EF-P alters the ribosome affinity to aminoacyl-tRNA. EF-1 γ acts during the delivery of aminoacyl tRNA to the ribosome. EF-2 promotes the translocation of the nascent protein chain from the A site to the P site on the ribosome. EF-3 is a unique EF in fungi hence it provides an anti-fungal drug target. See HEAT Repeat EF Spt4, Spt5, Spt6 are conserved among eukaryotes. They modulate the chromatin structure. EF-CA150 is believed to play a role in coupling transcription and splicing. Elongin complex or SIII activates elongation by RNA polymerase II by suppressing transient pausing of the enzyme. The complex is composed of elongin A, B and C. Elongin A (EloA) is the active component of the complex. Elongin B and C (EloBC) are the regulatory subunits of it. Von Hippel-Landau tumor suppressor protein (VHL) binds to EloBC and inhibits transcriptional elongation. Complex EF-Tu with EF-Ts is heterotetramer, or, more exactly heterodimer of homodimers (PDB entry 1efu).

3D structures of elongation factor

Updated on 11-March-2019

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• EF-Tu
  • SelB, HEAT Repeat, 1efu, 2fx3, 1etu, 5mi3 – EcEF – Escherichia coli
    
  • 5mi8, 5mi9 – EcEF (mutant)
    
  • 1qzd – EcEF – Cryo EM
    
  • 3u2q – EcEF + drug
    
  • 1mj1 – EcEF + Phe-tRNA + S12 + S13 + L11 – Cryo EM
    
  • 3ep2 - EcEF + tRNA + S12 + L11 + 16SRRNA + 23SRRNA – Cryo EM
    
  • 1ttt, 1ob5 – TaEF + Phe-tRNA + GDPNP - Thermus aquaticus
    
  • 1b23 - TaEF + Cys-tRNA
    
  • 1ls2 – yEF + Phe-tRNA – yeast – Cryo EM
    
  • 3wxm – ApEF + plethora protein – Aeropyrum pernix
    
  • 4h9g, 4lbv, 4lbw, 4lby, 4lbz, 4lc0 – TtEF – Thermus thermophilus
    
  • 4j0q – EF – Pseudomonas putida
    
  • 5w75 – EF – Thermotoga neapolitana
    
• EF-Tu complex with antibiotics
  • 1ob2 - yEF + Phe-tRNA + antibiotic
    
  • 4v8q - TtEF + antibiotic in 30S ribosome
    
  • 1ha3 - TtEF + antibiotic + GDP
    
  • 2c77, 2c78 - TtEF + antibiotic + GTP analog
    
  • 4v68 – TtEF + antibiotic in 70S ribosome – Cryo EM
    
  • 2bvn - EcEF + GDP
    NP + antibiotic
    
  • 1d8t, 2hcj, 2hdn, 3u6b, 3u6k, 4g5g, 5jbq – EcEF + antibiotic
• EF-Tu complex with nucleotide
  • 1efu – EcEF + GDP
    
  • 1exm – TtEF + GTP analog
    
  • 1eft – TaEF + GNP – Thermus aquaticus
    
  • 1tui – TaEF + GDP
    
  • 1d2e – bEF + GDP – bovine
    
  • 1jny, 1skq – SsEF + GDP – Sulfolobus solfataricus
    
  • 3wya – PhEF + GDP - Pyrococcus horikoshii
    
• EF-Tu complex with protein
  • 3vmf – ApEF + translation termination factor
    
  • 3wy9 – PhEF + L12
    
  • 4p3y – EcEF + thiol:disulfide interchange protein
    
  • 5i4r, 5i4q – EcEF + CDIA + CDII
    
  • 4zv4 – PaEF + TSE6 - Pseudomonas aeruginosa
    
• EF-Tu with EF-Ts
  • 1efu, 3agp, 3agq, 4pc1 – EcEF + EcEF-Ts
    
  • 3avt, 3avu , 3avv, 3avw, 3avx, 3avy, 3vnu , 3vnv, 4fwt – EcEF + EcEF-Ts + RNA
    
  • 3mmp – EcEF + EcEF-Ts + RNA replicase beta chain
    
  • 4q7j – EcEF + EcEF-Ts + Q beta replicase + S1
    
  • 4r71 – EcEF + EcEF-Ts + RNA polymerase β chain + S1
    
  • 4pc7 – EcEF + EcEF-Ts + antibiotic + GNP
    
  • 4pc2, 4pc3, 4pc6 – EcEF + EcEF-Ts + GDP derivative
    
  • 1aip - TtEF + TtEF-Ts - Thermus thermophilus
    
  • 1xb2 – bEF + EF-Ts - bovine
    
  • 1f60 – yEF + EF-Ts C terminal
    
  • 1g7c - yEF + EF-Ts C terminal + GDPNP
    
  • 1ije, 1ijf - yEF + EF-Ts C terminal + GDP
    
  • 2b7b - yEF + EF-Ts C terminal (mutant) + GDP
    
  • 2b7c - yEF + EF-Ts C terminal (mutant)
• EF-Tu in the ribosome
  • 3eq3, 3eq4 – EcEF in 80S ribosome – Cryo EM
    
  • 4v6l, 4v6k, 4v69 - EcEF in 30S ribosome – Cryo EM
    
  • 1zc8, 3dwu – TtEF in 70S ribosome – Cryo EM
    
  • 4v5g, 4v5l, 4v5p, 4v5q, 4v5r, 4v5s - TtEF in 70S ribosome
• EF-SII
  • 1tfi – hEF – human – NMR
    
  • 3ndq - hEF domain II
    
  • 1enw – yEF domain II – NMR
    
  • 1eo0 - yEF domain I – NMR
    
  • 2xex – SaEF – Staphylococcus aureus
    
  • 1pqv, 1y1v – yEF + RNA polymerase II
    
  • 1y1y, 3gtm – yEF + RNA polymerase II + RNA
    
  • 1wjt – mEF N terminal – mouse – NMR
    
  • 2m1h – EF PWWP domain – Trypanosoma brucei – NMR
    
• EF-ELL2
  • 2e5n – hEF N2 domain - NMR
    
  • 5jw9 – hEF + AFF4 peptide
    
• EF-G
  • 1efg, 1elo, 1ktv, 1wdt, 2dy1 – TtEF
    
  • 4m1k, 4myt, 4myu – TtEF (mutant)
    
  • 1pn6, 3izp – TtEF – Cryo EM
    
  • 1fnm, 2bm0, 2bm1 – TtEF (mutant)
    
  • 2bcw – TtEF + L11 + L7/L12
    
  • 2xex – SaEF
    
  • 3zz0, 3zzt, 3zzu – SaEF (mutant)
    
  • 2bv3 - TtEF (mutant) + GTP analog
    
  • 2j7k - TtEF (mutant) + GDP analog
    
  • 1dar, 2efg – TtEF + GDP
    
  • 1jqm, 1jqs – EcEF + L11 – Cryo EM
    
  • 4fn5 – PaEF
    
  • 5tv2 – EF – Vibrio vulnificus
    
  • 5ty0 – EF N terminal – Legionella pneumophila
    
  • 5vh6 – EF N terminal – Bacillus subtilis
    
  • 6b8d – EF N terminal – Haemophilus influenzae
    
• EF-G in the ribosome
  • 4v8u, 4v5m, 4v5n – TtEF in 70S ribosome
    
  • 4v9h, 4v9j, 4v9k, 4v9l, 4v9m, 4v90 – TtEF in 30S ribosome
    
  • 4v9o, 4v9p – EcEF in 30S ribosome
    
  • 4v9d – EcEF in 30S ribosome + antibiotics – Cryo-EM
    
  • 2om7, 4v5f – TtEF in 70S ribosome – Cryo EM
    
  • 1zn0, 2rdo, 3j0e, 4v6t, 4v9b – EcEF in 30S ribosome – Cryo-EM
    
• EF-GreA/GreB
  • 2pn0 – EF – Nitrosomonas europaea
    
  • 2p4v – EcEF-GreB
    
  • 1grj – EcEF-GreA
    
• EF-NusA
  • 1wcl, 1wcn – EcEF C terminal – NMR
    
  • 2kwp - EcEF N terminal – NMR
    
  • 2jzb – EcEF + RNA polymerase subunit α - NMR
    
• EF-P
  • 1ueb – TtEF
    
  • 4v6a – TtEF in 70S ribosome
    
  • 3oyy – PaEF
    
  • 3tre – EF – Coxiella burnetii
    
  • 3a5z – EcEF + lysyl-tRNA synthetase
    
  • 1yby – EF – Clostridium thermocellum
    
  • 5j3b – EF – Acinetobacter baumannii
    
  • 5wxk – EF + EARP – Neisseria meningitidis
    
• EF-Ts
  • 1tfe – TtEF
    
  • 1b64 – hEF guanine exchange factor domain – NMR
    
  • 2cp9 – UBA domain – NMR
    
  • 1gh8 – EF – Methanobacterium thermoautotrophicum – NMR
    
  • 2yy3 – PhEF
    
  • 2uz8 – hEF (mutant)
• EF-1
  • 4c0s – rEF α-2 – rabbit
    
  • 5dqs – hEF β+γ
    
  • 5jpo – hEF γ+δ
    
  • 1pbu – hEF γ C terminal (mutant) - NMR
    
  • 2mvm, 2mvn – hEF δ-1 CAR domain – NMR
    
  • 5o8w – yEF α+β
    
  • 1nhy – yEF γ N terminal
    
• EF-2
  • 1n0v – yEF
    
  • 1u2r – yEF + GDP
    
  • 1n0u, 2e1r, 2npf – yEF + antifungal drug
    
  • 1zm2, 1zm3, 1zm4, 1zm9, 3b78, 3b82, 3b8h, 2zit – yEF + exotoxin
    
  • 4v4b - hEF in 40S ribosome – Cryo EM
    
  • 4cxg, 4cxh - rEF in 40S ribosome – Cryo EM
    
  • 2p8w, 2p8x, 3dny - yEF in 80S ribosome – Cryo EM
    
  • 2p8y, 2p8z - yEF+ antifungal drug in 80S ribosome – Cryo EM
    
  • 5h7j - PhEF
    
  • 5h7l - PhEF + L12
    
  • 5h7k - PhEF + GDP
    
• EF-3
  • 2ix3 – yEF
    
  • 2iwh – yEF + ADPNP
    
  • 2iw3 – yEF + ADP
    
  • 2ix8 – yEF in 80S ribosome – Cryo EM
    
• EF-4 (lepA)
  • 2ywh, 2ywe, 2ywf, 2ywg - AalepA + nucleotide - Aquifex aeolicus
    
  • 3cb4 - EclepA
    
  • 3deg - EclepA in 70S ribosome + GMPPNP – Cryo EM
    
• EF-SelB
  • 1lva – MtEF C terminal – Moorella thermoacetica
    
  • 2v9v – MtEF winged helix domain
    
  • 1wsu, 2uwm – MtEF + RNA
    
  • 2ply – MtEF (mutant) + RNA
    
  • 4zu9 - AaEF
    
  • 4aca – MmEF – Methanococcus maripaludis
    
  • 4acb – MmEF + GTP analog
    
  • 4ac9 – MmEF + GDP
    
  • 2pjp – EcEF + RNA
    
  • 5izk, 5izl, 5izm – hEF + GDP derivative
    
• EF-Spt4 + EF-Spt5
  • 5xon, 5xog – EF-Spt4 + EF-Spt5 + EF-Tfiis + RNA polymerase II - Komagataella phaffii - Cryo EM
    
  • 5oik – bEF-Spt4 + EF-Spt5 + RNA polymerase II
    
  • 2exu – yEF-Spt4 residues 1-99 + EF-Spt5 residues 285-375
    
  • 3h7h – hEF-Spt4 residues 2-117 + hEF-Spt5 residues 176-273
    
  • 4zn3, 4zn1 – EF-Spt4 + EF-Spt5 – Methanocaldococcus jannaschii
    
• EF-Spt5
  • 2do3, 2e6z, 2e70 – hEF KOW motif residues 694-757 – NMR
    
  • 5ohq – hEF KOW6-KOW7 domain residues 939-1087
    
  • 5oho – hEF KOWX-KOW4 domain residues 536-646
    
  • 4ytl – yEF KOW2-KOW3 domain residues 534-632
    
  • 4ytk – yEF KOW1-linker domain residues 382-511
    
  • 2exu – yEF
    
• EF-Spt6
  • 3gxw, 3gxx, 3pjp – EF SH2 domain – Candida glabrata
    
  • 3psf, 3psi – yEF core domain residues 235-1259
    
  • 3psj, 3psk – yEF SH2 domain residues 1247-1451
    
  • 5vkl, 5vko – yEF SH2 domain + RPB1 peptide
    
  • 2l3t - yEF SH2 domain - NMR
    
  • 3oak – yEF + transcription factor IWS1
    
  • 4z2n – hEF middle domain residues 644-930
    
  • 4z2m – hEF middle domain + histone H3.1 + histone H4
    
• EF-Spt16
  • 5umu, 5umt – hEF
    
  • 5e5b - hEF N-terminal
    
• EF-M2-1
  • 5nkx, 5noh – EF core domain – human respiratory cyncytial virus
    
• EF-TEFM mitochondrial
  • 5ol9 - hEF N-terminal
    
  • 5ol8 - hEF C-terminal
    
  • 5ola - hEF + RNA polymerase + RNA + DNA
    
• EF-CA150
  • 2dod, 2doe, 2dof, 2e71, 2kiq, 2kis – hEF FF domain – NMR
    
  • 3hfh – hEF FF domain
    
  • 2ysi– mEF WW domain - NMR
    
• Elongin A

• • 4hfx – hEloA F-box domain
    
• Elongin BC complex
  • 1lqb, 1lm8 – hEloBC + von-Hippel Lindau disease tumor suppressor + hypoxia inducible factor 1 α
    
  • 3zrc, 3ztc, 3ztd, 3zun, 6fmi, 6fmj, 6fmk, 5nw2, 5nw1, 5nw0, 5nvz, 5nvy, 5nvx, 5nvw, 5nvv, 4w9l, 4w9k, 4w9j, 4w9i, 4w9h, 4w9g, 4w9f, 4w9e, 4w9d, 4w9c - hEloBC + von-Hippel Lindau disease tumor suppressor + inhibitor
    
  • 5t35 - hEloBC + von-Hippel Lindau disease tumor suppressor + BRD4
    
  • 4wqo - hEloBC + von-Hippel Lindau disease tumor suppressor + cullin-2
    
  • 5n4w - hEloBC + von-Hippel Lindau disease tumor suppressor + cullin-2 + RBX1
    
  • 3zrf, 5lli - hEloBC + von-Hippel Lindau disease tumor suppressor
    
  • 2c9w, 2izv, 2jz3, 5bo4 – hEloBC + suppressor of cytokine signaling
    
  • 6c5x – hEloBC + suppressor of cytokine signaling + Gp130 peptide
    
  • 3dcg – hEloBC + virion infectivity factor
    
  • 3zkj - hEloBC + ankyrin rep
    
  • 1vcb – hEloBC + VHL
    
  • 2fnj - mEloBC + GUSTAVUS

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