Gyrase

spinqualitylabelsall models Gyrase subunit A N-terminal (cyan) and subunit B C-terminal (magenta) complex with DNA, inhibitor, sulfate and Mn+2 ion (purple) (PDB entry 4plb) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Function Gyrase (Gyr) is a type of topoisomerase II in prokaryotes which unwinds double stranded DNA. The DNA Gyr cutting allows the formation of a negative DNA supercoil which enables replication of DNA Gyr consists of 2 subunits: GyrA and GyrB. Reverse gyrase (Top-RG) is a type of topoisomerase I which catalyses the formation of positive DNA supercoil. See also Isomerases. Relevance GyrA inhibitor Ciprofloxacin is used as antibiotic drug. Fluoroquinolones are Gyr inhibitors used in treatment of multi drug-resistant tuberculosis Structural highlights Gyrase subunit A N-terminal and subunit B C-terminal complex with DNA and inhibitor. A potential drug interacts with both DNA strands and in the hydrophobic pocket between subunit A and B. Water molecules shown as red spheres.

3D Structure of Gyrase

Updated on 10-June-2018

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• Gyrase Subunit A
  • Isomerases – GyrA C-terminal – Xanthomonas campestris
    
  • Ciprofloxacin, 4ckl – EcGyrA N-terminal+simocylinone – Escherichia coli
    
  • 1ajb - EcGyrA N-terminal+novobiocin
    
  • 4ckk - EcGyrA N-terminal
    
  • 1zi0, 1ab4 - EcGyrA C-terminal
    
  • 1x75 – EcGyrA14+CcdB
    
  • 4elz - GyrA fragment+CcdB – Vibrio fischeri
    
  • 3ilw, 3ifz - MtGyrA ATPase domain – Mycobacterium tuberculosis
    
  • 3uc1, 4g3n - MtGyrA C-terminal
    
  • 1suu - GyrA C-terminal – Borrelia burgdorferi
    
  • 3no0 - GyrA C-terminal – Aquifex aeolicus
    
  • 3lpx – GyrA N-terminal – Colwellia psychrerithraea
    
  • 4ely – GyrA residues 363-497 + CcdB – Shigella flexneri
    
  • 4ddq – GyrA – Bacillus subtilis
    
• Gyrase Subunit B
  • 3g75, 3g7b, 3g7e, 3ttz, 3u2d, 3u2k, 4p8o, 5cph, 5ctu, 5ctw, 5ctx, 5cty – GyrB ATP-binding domain residues 2-234 + inhibitor – Staphylococcus aureus
    
  • 5d6p, 5d6q, 5d7c, 5d7d, 5d7r - SaGyrB ATP-binding domain + ligand
    
  • 5npk, 5npp - SaGyrB residues 410-1491 + DNA
    
  • 5mmn, 5mmo, 5mmp - EcGyrB ATP-binding domain + antibacterial
    
  • 4urm, 4uro - SaGyrB N-terminal + antibiotic
    
  • 2zjt, 3ig0, 3m4i - MtGyrB C-terminal
    
  • 3cwv – GyrB truncated – Myxococcus xanthus
    
  • 4wub, 4wuc, 4wud, 4xtj - EcGyrB N-terminal
    
  • 1kzn, 1ei1, 1aj6 - EcGyrB N-terminal+ antibiotic
    
  • 4duh, 4zvi, 5l3j - EcGyrB N-terminal+ inhibitor
    
  • 4prv, 4prx, 4pu9 - EcGyrB N-terminal+ ADP
    
  • 4hyp - EcGyrB residues 15-220 + inhibitor
    
  • 1kij – GyrB ATPase domain+ antibiotic – Thermus thermophilus
    
  • 4b6c - GyrB ATPase domain – Mycobacterium smegmatis
    
  • 4gee, 4gfn, 4ggl, 4hxw, 4k4o, 4kfg, 4ksg, 4ksh, 4ktn – GyrB + inhibitor – Enterococcus faecalis
    
  • 3zkb, 3zkd - MtGyrB ATPase domain + AMPPNP
    
  • 3zm7 - MtGyrB ATPase domain + AMPPCP
    
  • 4bae - MtGyrB ATPase domain (mutant) + inhibitor
    
• Gyrase Subunit A+Subunit B
  • 2xco, 2xcq - SaGyrB C-terminal/SaGyrA N-terminal
    
  • 4plb - SaGyrB C-terminal/SaGyrA N-terminal +DNA
    
  • 2xcr, 2xcs, 4bul - SaGyrB C-terminal/SaGyrA N-terminal (mutant)+DNA
    
  • 2xct - SaGyrB C-terminal/SaGyrA N-terminal (mutant) +DNA+ antibiotic
    
  • 4tma - SaGyrB C-terminal- + SaGyrA N-terminal + Gyr inhibitor YACG
    
  • 3nuh – EcGyrA+EcGyrB
    
  • 5cdn, 5cdo, 5cdp, 6fqv – SaGyrA + SaGyrB + DNA
    
  • 5cdq, 6fqm, 6fqs - SaGyrB + GyrA + DNA + antibiotic
    
  • 5cdm – SaGyrA + SaGyrB (mutant) + DNA
    
  • 5cdr – SaGyrA (mutant) + SaGyrB (mutant) + DNA
    
  • 4z2c, 4z2d - SpGyrB + GyrA + DNA – Streptococcus pneumoniae
    
  • 4z2e - SpGyrB + GyrA + DNA + antibiotic
    
  • 5bs3, 5iwi, 5iwm - SaGyrB + GyrA + DNA + inhibitor
    
  • 5bs8, 5bta, 5btd, 5btg, 5btl - MtGyrB + GyrA + DNA + antibiotic
    
  • 5btc, 5btf, 5bti, 5btn - MtGyrB + GyrA (mutant) + DNA + antibiotic
    
• Reverse Gyrase
  • 1gku – AfTop-RG – Archaeoglobus fulgidus
    
  • 1gl9 - AfTop-RG+ADPNP
    
  • 3oiy, 3p4y – TmTop-RG helicase domain – Thermotoga maritima
    
  • 4ddt, 4ddu, 4ddv, 4ddw, 4ddx - TmTop-RG

Additional Resources

For additional information, see: Bacterial Infections

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