Sandbox CsrA

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Contents 1 CsrA 1.1 Residues Highlighted by Alanine Scanning Mutation of CsrA[2] 2 E. coli CsrA 2.1 Residues Highlighted by Alanine Scanning Mutation of CsrA[2] 3 Aligned CsrA structures 3.1 Residues Highlighted by Alanine Scanning Mutation of CsrA[2] 3.2 Aligned Structures 4 Model of Vibrio CsrA 4.1 Residues Highlighted by Alanine Scanning Mutation of CsrA[2] 5 Aligned CsrA structures and Vibrio Model 5.1 Residues Highlighted by Alanine Scanning Mutation of CsrA[2] 5.2 Aligned Structures 6 Structures Used On This Page 7 See Also 8 References

CsrA

spinqualitylabelsall models CsRA (Pseudomonas aeruginosa) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Residues 24-27 form a ^[1].

Residues Highlighted by Alanine Scanning Mutation of CsrA^[2]

 

 

 

 

 

 

 

 

E. coli CsrA

spinqualitylabelsall models CsRA (E. coli) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Residues Highlighted by Alanine Scanning Mutation of CsrA^[2]

 

 

 

 

 

 

 

 

Aligned CsrA structures

spinqualitylabelsall models CsRA structures aligned Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Residues Highlighted by Alanine Scanning Mutation of CsrA^[2]

Aligned Structures

Display .

(E. coli)

(Pseudomonas aeruginosa)

(Yersinia enterocolitica)

 

Display .

(E. coli)

(Pseudomonas aeruginosa)

(Yersinia enterocolitica)
 

 

 

 

 

 

 

 

Model of Vibrio CsrA

Theoretical Model: The protein structure described in this section was determined theoretically, and hence should be interpreted with caution.

spinqualitylabelsall models CsRA (V. fischeri) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Residues Highlighted by Alanine Scanning Mutation of CsrA^[2]

 

 

 

 

 

 

 

 

Aligned CsrA structures and Vibrio Model

Theoretical Model: The Vibrio structure described in this section was determined theoretically, and hence should be interpreted with caution.

spinqualitylabelsall models CsRA structures aligned Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Residues Highlighted by Alanine Scanning Mutation of CsrA^[2]

Aligned Structures

Display .

(E. coli)

(Pseudomonas aeruginosa)

(Yersinia enterocolitica)

(Vibrio fischeri)

 

Display .

(E. coli)

(Pseudomonas aeruginosa)

(Yersinia enterocolitica)

(Vibrio fischeri)

Structures Used On This Page

1y00 is a 2 chains structure of sequences from Escherichia coli. Full experimental information is available from OCA.

1vpz is a 2 chains structure of sequences from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.

2bti is a 2 chains structure of sequences from Yersinia enterocolitica. Full crystallographic information is available from OCA.

See Also

1t3o - NMR structure of Bacillus subtilis CsrA

References

1. ↑ Rife C, Schwarzenbacher R, McMullan D, Abdubek P, Ambing E, Axelrod H, Biorac T, Canaves JM, Chiu HJ, Deacon AM, DiDonato M, Elsliger MA, Godzik A, Grittini C, Grzechnik SK, Hale J, Hampton E, Han GW, Haugen J, Hornsby M, Jaroszewski L, Klock HE, Koesema E, Kreusch A, Kuhn P, Lesley SA, Miller MD, Moy K, Nigoghossian E, Paulsen J, Quijano K, Reyes R, Sims E, Spraggon G, Stevens RC, van den Bedem H, Velasquez J, Vincent J, White A, Wolf G, Xu Q, Hodgson KO, Wooley J, Wilson IA. Crystal structure of the global regulatory protein CsrA from Pseudomonas putida at 2.05 A resolution reveals a new fold. Proteins. 2005 Nov 1;61(2):449-53. PMID:16104018 doi:10.1002/prot.20502
2. ↑ ^{2.0 2.1 2.2 2.3 2.4} Mercante J, Suzuki K, Cheng X, Babitzke P, Romeo T. Comprehensive alanine-scanning mutagenesis of Escherichia coli CsrA defines two subdomains of critical functional importance. J Biol Chem. 2006 Oct 20;281(42):31832-42. Epub 2006 Aug 21. PMID:16923806 doi:10.1074/jbc.M606057200