Kisker lab: 5B5Q

From Proteopedia

(Redirected from User:Karsten Theis/5B5Q)
Jump to: navigation, search
See User added comments


How this page was created

The goal of this page is to provide three-dimensional and interactive figures to explore the structure of a protein involved in bacterial infections (PDB code 5B5Q). The starting point are the figures found in the paper describing the 5B5Q structure. Biochemistry students from Westfield State University recreated these figures as best as possible in jmol, and revised them after getting feedback from researchers who authored the primary citation (Kisker lab in Würzburg, Germany). A special thank you goes to Ose Aimua, Nina Aldabayeva, Faiqa Ashraf, Kaleigh Florek, Ellie Hoeg, Aya Maytham, Christian Mikule, Brigid Murray, Kevin Pelletier, Brandon Reder, Erin Riley, Brian Schuler, and Jakob Wyman for the revisions of figures and for working on the links to other proteopedia pages.

If you are interested to see the jmol scripts used to make these figures, take a look at the discussion page (2nd tab above).

Chlamydia trachomatis inhibits apoptosis

Chlamydia trachomatis is a bacterium that reproduces inside human cells. One defense of the human body against Chlamydia is to kill affected human cells before Chlamydia reproduces and infects more human cells. This is done through a process called apoptosis, programmed cell death. One player in apoptosis is the human protein Mcl-1. High Mcl-1 levels inhibit one of the signalling pathways that lead to apoptosis. Chlamydia inhibits Mcl-1 degradation so that Mcl-1 levels remain high.

Protein ubiquitination and degradation

Human cells have a protein assembly called the proteasome, which specializes in degrading proteins. Ubiquitin is a small, highly soluble protein; when ubiquitin chains are attached to other proteins in a certain way, it acts as a signal for protein degradation. The proteasome only degrades proteins that are poly-ubiquitinated, i.e. are covalently linked to a linear chain of ubiquitins. The covalent link is between the amino group of a lysine side chain and the carboxylic acid of a glycine at the C-terminus of ubiquitin.

The deubiquitinase activity of Cdu1 stabilizes Mcl-1

The Chlamydia protein Cdu1 is a protease that catalyzes the hydrolysis of ubiquitin chains from Mcl-1. When polyubiquitinated, Mcl-1 is destined to be degraded by the proteasome, lowering the level of Mcl-1 and subsequently leading to apoptosis. The activity of Cdu1 counteracts this by removing the ubiquitin, thus leading to higher levels of Mcl-1 in the cell.


Caption for this structure

Drag the structure with the mouse to rotate

Relevant Links

5b5q : autogenerated Proteopedia page on coordinates

Ubiquitin Structure & Function : Proteopedia article on Ubiquitin Structure, function and conjugation

Ubiquitin chains : Proteopedia article on Ubiquitin Chains

Category: Kisker C : list of structures associated with the Kisker lab

Proteopedia Page Contributors and Editors (what is this?)

Karsten Theis

Personal tools