VirE1-VirE2

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Crystal structure of the Agrobacterium virulence complex VirE1-VirE2 reveals a flexible protein that can accommodate different partners

Orly Dym, Shira Albeck, Tamar Unger, Jossef Jacobovitch, Anna Branzburg, Yigal Michael, Daphna Frenkiel-Krispin, Sharon Grayer Wolf, and Michael Elbaum [1]


      The soil bacterium Agrobacterium tumefaciens is a natural pathogen with the unique ability to genetically transform plants resulting in the Crown Gall disease. In addition to DNA, the bacterium delivers an abundant ssDNA binding protein, VirE2, whose roles in the host include protection from cytoplasmic nucleases and adaptation for nuclear import. The structure of VirE2, is presented here in complex with its natural acidic chaperone VirE1. VirE2 exhibits two structurally similar domains, each of which presents a combination of TIM barrel architecture with a Beta-Beta-Alpha-Alpha topology. This combination defines the novel VirE2 fold. The structural similarity between the two domains is remarkable (RMSD of 3.1Å) considering the very low sequence homology between them (14.3%). Also notable is that the two domains are connected by a single, extended linker (337-346) which exhibits no electron density for residues 343 and 344 suggesting that it is flexible. In the heterodimer, the two folded domains of VirE2 clamp. tightly around the single alpha-helix of VirE1. Both electrostatic and hydrophobic interactions with VirE1 cement the two domains of VirE2 into a “locked” conformation where the flexible extended linker joining these two independent VirE2 domains does not constrain their relative orientation. Most of the interactions are electrostatic, involving salt bridges between residues R168, K248, H315, R367 and K471 from VirE2 and N34, D40, E42, E45, E47 and N48 from VirE1. The acidic residues of VirE1 contribute to its strong electronegative surface resembling that of ssDNA. VirE2 can bind alternatively to VirE1 or to ssDNA. The acidic nature common to both substrates suggests that they bind via electrostatic interactions to a common region of VirE2. The two VirE2 domains also contain clusters of aromatic residues with side chains pointing toward the VirE1-binding site forming hydrophobic pocket. These aromatic residues include Y160, Y194, Y373, Y319, W323 and W383, with the last three being involved in VirE1 interactions. These planar side chains may form additional stacking interactions with the nucleotide bases of DNA. This analysis does not rule out additional interactions between ssDNA and parts of VirE2 that do not interact with VirE1. It appears that the two domains of VirE2 acquire a different relative orientation upon binding of VirE1 versus ssDNA utilizing conformational changes enabled by the extended flexible linker. In the presence of ssDNA, VirE2 adopts a solenoidal form whose three-dimensional structure has been characterized previously using electron microscopy (EM) and a real-space helical image processing approach[2]. A comparison of the crystal structure of VirE2 in complex with VirE1, to the EM reconstruction of VirE2 in the presence of ssDNA, supports rearrangement of the VirE2 domains. Thus VirE2 appears as a dynamic structure that can accommodate its different partners due to the flexibility of its inter-domain linker. These structural features reflect the physiological requirements of VirE1 as an inhibitory chaperone of VirE2 in the bacterium, and of VirE2 as a chaperone of the ssDNA in the plant.

Crystal structure of VirE2 in complex with VirE1 (dark blue) (3btp)

Drag the structure with the mouse to rotate

See also [[DNA-binding protein VirE2 from Agrobacterium tumefaciens complexed with chaperone VirE1]].

3D structures of VirE1-VirE2

Updated on 11-January-2021

3btp - AtVirE1-VirE2 - Agrobacterium tumefaciens
4blf - AtVirE2 - Cryo EM

References

  1. Dym O, Albeck S, Unger T, Jacobovitch J, Branzburg A, Michael Y, Frenkiel-Krispin D, Wolf SG, Elbaum M. Crystal structure of the Agrobacterium virulence complex VirE1-VirE2 reveals a flexible protein that can accommodate different partners. Proc Natl Acad Sci U S A. 2008 Aug 12;105(32):11170-5. Epub 2008 Aug 4. PMID:18678909
  2. Abu-Arish A, Frenkiel-Krispin D, Fricke T, Tzfira T, Citovsky V, Wolf SG, Elbaum M. Three-dimensional reconstruction of Agrobacterium VirE2 protein with single-stranded DNA. J Biol Chem. 2004 Jun 11;279(24):25359-63. Epub 2004 Mar 30. PMID:15054095 doi:10.1074/jbc.M401804200

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