1v1q

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(New page: 200px<br /> <applet load="1v1q" size="450" color="white" frame="true" align="right" spinBox="true" caption="1v1q, resolution 2.10&Aring;" /> '''CRYSTAL STRUCTURE O...)
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==About this Structure==
==About this Structure==
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1V1Q is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with CYS and CYS as [[http://en.wikipedia.org/wiki/ligands ligands]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1V1Q OCA]].
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1V1Q is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with CYS and CYS as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1V1Q OCA]].
==Reference==
==Reference==
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[[Category: primosome]]
[[Category: primosome]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 19:29:34 2007''
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:36:20 2007''

Revision as of 10:31, 30 October 2007

Image:1v1q.gif

1v1q, resolution 2.10Å

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CRYSTAL STRUCTURE OF PRIB- A PRIMOSOMAL DNA REPLICATION PROTEIN OF ESCHERICHIA COLI

Overview

PriB is one of the Escherichia coli varphiX-type primosome proteins that, are required for assembly of the primosome, a mobile multi-enzyme complex, responsible for the initiation of DNA replication. Here we report the, crystal structure of the E. coli PriB at 2.1 A resolution by, multi-wavelength anomalous diffraction using a mercury derivative. The, polypeptide chain of PriB is structurally similar to that of, single-stranded DNA-binding protein (SSB). However, the biological unit of, PriB is a dimer, not a homotetramer like SSB. Electrophoretic mobility, shift assays demonstrated that PriB binds single-stranded DNA and, single-stranded RNA with comparable affinity. We also show that PriB binds, single-stranded DNA with certain base preferences. Based on the PriB, structural information ... [(full description)]

About this Structure

1V1Q is a [Single protein] structure of sequence from [Escherichia coli] with CYS and CYS as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

Crystal structure of PriB, a primosomal DNA replication protein of Escherichia coli., Liu JH, Chang TW, Huang CY, Chen SU, Wu HN, Chang MC, Hsiao CD, J Biol Chem. 2004 Nov 26;279(48):50465-71. Epub 2004 Sep 21. PMID:15383524

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