Mandelate dehydrogenase

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<StructureSection load='2a7p' size='400' side='right' caption='Structure of S-mandelate dehydrogenase complex with 3-indoleacetate and flavin mononucleotide (PDB entry [[2a7p]])' scene=''>
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<StructureSection load='2a7p' size='450' side='right' caption='Structure of S-mandelate dehydrogenase tetramer complex with 3-indolelactate and flavin mononucleotide (PDB entry [[2a7p]])' scene='51/516477/Cv/1'>
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== Function ==
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'''S-mandelate dehydrogenase''' (SMD) catalyzes the conversion of S-2-hydroxy-2-phenylacetate and acceptor to 2-oxo-2-phenylacetate and reduced acceptor. SMD is a FMN-dependent enzyme.
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'''S-mandelate dehydrogenase''' (SMD) catalyzes the conversion of S-2-hydroxy-2-phenylacetate and acceptor to 2-oxo-2-phenylacetate and reduced acceptor<ref>PMID:10231535</ref>. SMD is a FMN-dependent enzyme. '''D-mandelate dehydrogenase''' (DMD) catalyzes the NAD-dependent oxidation of D-mandalate to phenylglyoxylate.
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== Structural highlights ==
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The biological assembly of SMD from ''Pseudomonas putida'' is <scene name='51/516477/Cv/7'>homotetramer</scene>. The <scene name='51/516477/Cv/8'>poor substrate indolelactate binds in the active site</scene> of SMD<ref>PMID:19465768</ref>. Water molecules shown as red spheres.
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</StructureSection>
==3D structures of mandelate dehydrogenase==
==3D structures of mandelate dehydrogenase==
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[[1huv]], [[1p4c]], [[1p5b]], [[2a7n]], [[3giy]] – PpSMD (mutant) – ''Pseudomonas putida''<br />
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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[[2w2l]] – SMD – ''Rhodotorula graminis''<br />
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{{#tree:id=OrganizedByTopic|openlevels=0|
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[[2a7p]] – PpSMD (mutant) + 3-indolelacetate<br />
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*S-mandelate dehydrogenase
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[[2a85]] – PpSMD (mutant) + 2-hydroxyoctanoate
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</StructureSection>
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**[[6bfg]] – PpSM + FMN - ''Pseudomonas putida''<br />
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**[[1huv]], [[1p4c]], [[1p5b]], [[2a7n]], [[3giy]] – PpSM (mutant) + FMN <br />
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**[[2a7p]] – PpSMD (mutant) + FMN + 3-indolelacetate<br />
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**[[2a85]] – PpSMD (mutant) + FMN + 2-hydroxyoctanoate<br />
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*D-mandelate dehydrogenase
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**[[2w2k]] – RgDMD – ''Rhodotorula graminis''<br />
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**[[2w2l]] – RgDMD + NAD <br />
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**[[3wfi]] – EfDMD – ''Enterococcus faecium''<br />
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**[[3wfj]] – EfDMD + NAD <br />
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**[[5x20]] – EfDMD + NAD + aniline(oxo)acetate <br />
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}}
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== References ==
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<references/>
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[[Category:Topic Page]]

Current revision

Structure of S-mandelate dehydrogenase tetramer complex with 3-indolelactate and flavin mononucleotide (PDB entry 2a7p)

Drag the structure with the mouse to rotate

3D structures of mandelate dehydrogenase

Updated on 26-June-2023

References

  1. ↑ Lehoux IE, Mitra B. (S)-Mandelate dehydrogenase from Pseudomonas putida: mechanistic studies with alternate substrates and pH and kinetic isotope effects. Biochemistry. 1999 May 4;38(18):5836-48. PMID:10231535 doi:http://dx.doi.org/10.1021/bi990024m
  2. ↑ Sukumar N, Dewanti A, Merli A, Rossi GL, Mitra B, Mathews FS. Structures of the G81A mutant form of the active chimera of (S)-mandelate dehydrogenase and its complex with two of its substrates. Acta Crystallogr D Biol Crystallogr. 2009 Jun;65(Pt 6):543-52. Epub 2009, May 15. PMID:19465768 doi:10.1107/S0907444909010270

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

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