Mandelate dehydrogenase
From Proteopedia
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| - | <StructureSection load='2a7p' size=' | + | <StructureSection load='2a7p' size='450' side='right' caption='Structure of S-mandelate dehydrogenase tetramer complex with 3-indolelactate and flavin mononucleotide (PDB entry [[2a7p]])' scene='51/516477/Cv/1'> |
== Function == | == Function == | ||
'''S-mandelate dehydrogenase''' (SMD) catalyzes the conversion of S-2-hydroxy-2-phenylacetate and acceptor to 2-oxo-2-phenylacetate and reduced acceptor<ref>PMID:10231535</ref>. SMD is a FMN-dependent enzyme. '''D-mandelate dehydrogenase''' (DMD) catalyzes the NAD-dependent oxidation of D-mandalate to phenylglyoxylate. | '''S-mandelate dehydrogenase''' (SMD) catalyzes the conversion of S-2-hydroxy-2-phenylacetate and acceptor to 2-oxo-2-phenylacetate and reduced acceptor<ref>PMID:10231535</ref>. SMD is a FMN-dependent enzyme. '''D-mandelate dehydrogenase''' (DMD) catalyzes the NAD-dependent oxidation of D-mandalate to phenylglyoxylate. | ||
== Structural highlights == | == Structural highlights == | ||
| - | The poor substrate indolelactate binds in the active site of SMD<ref>PMID:19465768</ref>. | + | The biological assembly of SMD from ''Pseudomonas putida'' is <scene name='51/516477/Cv/7'>homotetramer</scene>. The <scene name='51/516477/Cv/8'>poor substrate indolelactate binds in the active site</scene> of SMD<ref>PMID:19465768</ref>. Water molecules shown as red spheres. |
</StructureSection> | </StructureSection> | ||
==3D structures of mandelate dehydrogenase== | ==3D structures of mandelate dehydrogenase== | ||
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*S-mandelate dehydrogenase | *S-mandelate dehydrogenase | ||
| - | **[[1huv]], [[1p4c]], [[1p5b]], [[2a7n]], [[3giy]] – PpSM + FMN | + | **[[6bfg]] – PpSM + FMN - ''Pseudomonas putida''<br /> |
| + | **[[1huv]], [[1p4c]], [[1p5b]], [[2a7n]], [[3giy]] – PpSM (mutant) + FMN <br /> | ||
**[[2a7p]] – PpSMD (mutant) + FMN + 3-indolelacetate<br /> | **[[2a7p]] – PpSMD (mutant) + FMN + 3-indolelacetate<br /> | ||
**[[2a85]] – PpSMD (mutant) + FMN + 2-hydroxyoctanoate<br /> | **[[2a85]] – PpSMD (mutant) + FMN + 2-hydroxyoctanoate<br /> | ||
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**[[2w2k]] – RgDMD – ''Rhodotorula graminis''<br /> | **[[2w2k]] – RgDMD – ''Rhodotorula graminis''<br /> | ||
**[[2w2l]] – RgDMD + NAD <br /> | **[[2w2l]] – RgDMD + NAD <br /> | ||
| + | **[[3wfi]] – EfDMD – ''Enterococcus faecium''<br /> | ||
| + | **[[3wfj]] – EfDMD + NAD <br /> | ||
| + | **[[5x20]] – EfDMD + NAD + aniline(oxo)acetate <br /> | ||
}} | }} | ||
== References == | == References == | ||
Revision as of 08:26, 26 June 2023
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3D structures of mandelate dehydrogenase
Updated on 26-June-2023
References
- ↑ Lehoux IE, Mitra B. (S)-Mandelate dehydrogenase from Pseudomonas putida: mechanistic studies with alternate substrates and pH and kinetic isotope effects. Biochemistry. 1999 May 4;38(18):5836-48. PMID:10231535 doi:http://dx.doi.org/10.1021/bi990024m
- ↑ Sukumar N, Dewanti A, Merli A, Rossi GL, Mitra B, Mathews FS. Structures of the G81A mutant form of the active chimera of (S)-mandelate dehydrogenase and its complex with two of its substrates. Acta Crystallogr D Biol Crystallogr. 2009 Jun;65(Pt 6):543-52. Epub 2009, May 15. PMID:19465768 doi:10.1107/S0907444909010270
