2qpf

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[[Image:2qpf.jpg|left|200px]]<br /><applet load="2qpf" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2qpf, resolution 2.05&Aring;" />
 
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'''Crystal Structure of Mouse Transthyretin'''<br />
 
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==Overview==
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==Crystal Structure of Mouse Transthyretin==
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The transthyretin amyloidoses appear to be caused by rate-limiting, tetramer dissociation and partial monomer unfolding of the human serum, protein transthyretin, resulting in aggregation and extracellular, deposition of amorphous aggregates and amyloid fibrils. Mice transgenic, for few copies of amyloid-prone human transthyretin variants, including, the aggressive Leu55Pro mutant, failed to develop deposits. Silencing the, murine transthyretin gene in the presence of the Leu55Pro human gene, resulted in enhanced tissue deposition. To test the hypothesis that the, murine protein interacted with human transthyretin, preventing the, dissociation and partial unfolding required for amyloidogenesis, we, produced recombinant murine transthyretin and human/murine transthyretin, heterotetramers and compared their structures and biophysical properties, to recombinant human transthyretin. We found no significant differences, between the crystal structures of murine and human homotetramers. Murine, transthyretin is not amyloidogenic because the native homotetramer is, kinetically stable under physiologic conditions and cannot dissociate into, partially unfolded monomers, the misfolding and aggregation precursor., Heterotetramers composed of murine and human subunits are also kinetically, stable. These observations explain the lack of TTR deposition in, transgenics carrying a low copy number of human transthyretin genes. The, incorporation of mouse subunits into tetramers otherwise composed of human, amyloid-prone transthyretin subunits imposes kinetic stability, preventing, dissociation and subsequent amyloidogenesis. The results demonstrate that, interspecies oligomers may have different biophysical characteristics than, the corresponding homo-oligomers and suggest that the elimination of the, endogenous protein might be required to obtain the desired disease, phenotype in transgenic models.
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<StructureSection load='2qpf' size='340' side='right'caption='[[2qpf]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2qpf]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QPF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QPF FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=OCS:CYSTEINESULFONIC+ACID'>OCS</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qpf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qpf OCA], [https://pdbe.org/2qpf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qpf RCSB], [https://www.ebi.ac.uk/pdbsum/2qpf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qpf ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TTHY_MOUSE TTHY_MOUSE] Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qp/2qpf_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qpf ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The transthyretin amyloidoses appear to be caused by rate-limiting tetramer dissociation and partial monomer unfolding of the human serum protein transthyretin, resulting in aggregation and extracellular deposition of amorphous aggregates and amyloid fibrils. Mice transgenic for few copies of amyloid-prone human transthyretin variants, including the aggressive L55P mutant, failed to develop deposits. Silencing the murine transthyretin gene in the presence of the L55P human gene resulted in enhanced tissue deposition. To test the hypothesis that the murine protein interacted with human transthyretin, preventing the dissociation and partial unfolding required for amyloidogenesis, we produced recombinant murine transthyretin and human/murine transthyretin heterotetramers and compared their structures and biophysical properties to recombinant human transthyretin. We found no significant differences between the crystal structures of murine and human homotetramers. Murine transthyretin is not amyloidogenic because the native homotetramer is kinetically stable under physiologic conditions and cannot dissociate into partially unfolded monomers, the misfolding and aggregation precursor. Heterotetramers composed of murine and human subunits are also kinetically stable. These observations explain the lack of transthyretin deposition in transgenics carrying a low copy number of human transthyretin genes. The incorporation of mouse subunits into tetramers otherwise composed of human amyloid-prone transthyretin subunits imposes kinetic stability, preventing dissociation and subsequent amyloidogenesis.
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==About this Structure==
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Human-murine transthyretin heterotetramers are kinetically stable and non-amyloidogenic. A lesson in the generation of transgenic models of diseases involving oligomeric proteins.,Reixach N, Foss TR, Santelli E, Pascual J, Kelly JW, Buxbaum JN J Biol Chem. 2008 Jan 25;283(4):2098-107. Epub 2007 Nov 15. PMID:18006495<ref>PMID:18006495</ref>
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2QPF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QPF OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Human-murine transthyretin heterotetramers are kinetically stable and non-amyloidogenic: A lesson in the generation of transgenic models of diseases involving oligomeric proteins., Reixach N, Foss TR, Santelli E, Pascual J, Kelly JW, Buxbaum JN, J Biol Chem. 2007 Nov 15;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18006495 18006495]
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</div>
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[[Category: Mus musculus]]
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<div class="pdbe-citations 2qpf" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Buxbaum, J.N.]]
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[[Category: Foss, T.R.]]
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[[Category: Kelly, J.W.]]
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[[Category: Pascual, J.]]
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[[Category: Reixach, N.]]
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[[Category: Santelli, E.]]
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[[Category: amyloidogenesis]]
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[[Category: glycoprotein]]
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[[Category: hormone]]
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[[Category: retinol-binding]]
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[[Category: secreted]]
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[[Category: tetramer]]
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[[Category: thyroid hormone]]
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[[Category: transport protein]]
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[[Category: vitamin a]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 6 17:28:50 2008''
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==See Also==
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*[[Transthyretin 3D structures|Transthyretin 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Mus musculus]]
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[[Category: Buxbaum JN]]
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[[Category: Foss TR]]
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[[Category: Kelly JW]]
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[[Category: Pascual J]]
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[[Category: Reixach N]]
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[[Category: Santelli E]]

Current revision

Crystal Structure of Mouse Transthyretin

PDB ID 2qpf

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Proteopedia Page Contributors and Editors (what is this?)

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