1hqh

From Proteopedia

(Difference between revisions)

Current revision

CRYSTAL STRUCTURE OF THE BINUCLEAR MANGANESE METALLOENZYME ARGINASE COMPLEXED WITH NOR-N-HYDROXY-L-ARGININE

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://proteopedia.org/wiki/index.php/1hqh"

@@ Line 1: / Line 1: @@
-[[Image:1hqh.jpg|left|200px]]<br /><applet load="1hqh" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1hqh, resolution 2.8&Aring;" />
-'''CRYSTAL STRUCTURE OF THE BINUCLEAR MANGANESE METALLOENZYME ARGINASE COMPLEXED WITH NOR-N-HYDROXY-L-ARGININE'''<br />
-==Overview==
+==CRYSTAL STRUCTURE OF THE BINUCLEAR MANGANESE METALLOENZYME ARGINASE COMPLEXED WITH NOR-N-HYDROXY-L-ARGININE==
-Arginase is a binuclear Mn(2+) metalloenzyme that catalyzes the hydrolysis, of L-arginine to L-ornithine and urea. X-ray crystal structures of, arginase complexed to substrate analogues N(omega)-hydroxy-L-arginine and, N(omega)-hydroxy-nor-L-arginine, as well as the products L-ornithine and, urea, complete a set of structural "snapshots" along the reaction, coordinate of arginase catalysis when interpreted along with the X-ray, crystal structure of the arginase-transition-state analogue complex, described in Kim et al. [Kim, N. N., Cox, J. D., Baggio, R. F., Emig, F., A., Mistry, S., Harper, S. L., Speicher, D. W., Morris, Jr., S. M., Ash, D. E., Traish, A. M., and Christianson, D. W. (2001) Biochemistry 40, 2678-2688]. Taken together, these structures render important insight on, the structural determinants of tight binding inhibitors. Furthermore, we, demonstrate for the first time the structural mechanistic link between, arginase and NO synthase through their respective complexes with, N(omega)-hydroxy-L-arginine. That N(omega)-hydroxy-L-arginine is a, catalytic intermediate for NO synthase and an inhibitor of arginase, reflects the reciprocal metabolic relationship between these two critical, enzymes of L-arginine catabolism.
+<StructureSection load='1hqh' size='340' side='right'caption='[[1hqh]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1hqh]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HQH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HQH FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NNH:NOR-N-OMEGA-HYDROXY-L-ARGININE'>NNH</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hqh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hqh OCA], [https://pdbe.org/1hqh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hqh RCSB], [https://www.ebi.ac.uk/pdbsum/1hqh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hqh ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/ARGI1_RAT ARGI1_RAT]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hq/1hqh_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hqh ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-HQH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with MN and NNH as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Arginase Arginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.3.1 3.5.3.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HQH OCA].
+*[[Arginase 3D structures|Arginase 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-Mechanistic and metabolic inferences from the binding of substrate analogues and products to arginase., Cox JD, Cama E, Colleluori DM, Pethe S, Boucher JL, Mansuy D, Ash DE, Christianson DW, Biochemistry. 2001 Mar 6;40(9):2689-701. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11258880 11258880]
+[[Category: Large Structures]]
-[[Category: Arginase]]
 [[Category: Rattus norvegicus]]
-[[Category: Single protein]]
+[[Category: Ash DE]]
-[[Category: Ash, D.E.]]
+[[Category: Cama E]]
-[[Category: Cama, E.]]
+[[Category: Christianson DW]]
-[[Category: Christianson, D.W.]]
+[[Category: Colleluori DM]]
-[[Category: Colleluori, D.M.]]
+[[Category: Cox JD]]
-[[Category: Cox, J.D.]]
-[[Category: MN]]
-[[Category: NNH]]
-[[Category: binuclear manganese cluster]]
-[[Category: substrate analogue]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 16:44:54 2007''

1hqh

From Proteopedia

Current revision

CRYSTAL STRUCTURE OF THE BINUCLEAR MANGANESE METALLOENZYME ARGINASE COMPLEXED WITH NOR-N-HYDROXY-L-ARGININE

Structural highlights

Function

Evolutionary Conservation

See Also

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox