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| - | [[Image:1kd4.gif|left|200px]] | |
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| - | {{Structure
| + | ==The Crystal Structure of r(GGUCACAGCCC)2, Barium form== |
| - | |PDB= 1kd4 |SIZE=350|CAPTION= <scene name='initialview01'>1kd4</scene>, resolution 1.85Å
| + | <StructureSection load='1kd4' size='340' side='right'caption='[[1kd4]], [[Resolution|resolution]] 1.85Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=BA:BARIUM+ION'>BA</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene>
| + | <table><tr><td colspan='2'>[[1kd4]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KD4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KD4 FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85Å</td></tr> |
| - | |GENE=
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BA:BARIUM+ION'>BA</scene></td></tr> |
| - | |DOMAIN=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kd4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kd4 OCA], [https://pdbe.org/1kd4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kd4 RCSB], [https://www.ebi.ac.uk/pdbsum/1kd4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kd4 ProSAT]</span></td></tr> |
| - | |RELATEDENTRY=
| + | </table> |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kd4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kd4 OCA], [http://www.ebi.ac.uk/pdbsum/1kd4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1kd4 RCSB]</span>
| + | __TOC__ |
| - | }}
| + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | '''The Crystal Structure of r(GGUCACAGCCC)2, Barium form'''
| + | [[Category: Kacer V]] |
| - | | + | [[Category: Rife JP]] |
| - | | + | [[Category: Scaringe SA]] |
| - | ==Overview==
| + | [[Category: Scarsdale JN]] |
| - | Crystals of small RNAs, which regularly diffract to very high resolution, can often be readily obtained. Unfortunately, for some RNAs the conformations adopted in the crystalline form are different from those found in solution. For example, short RNAs that form hairpins in solution virtually never crystallize thus; rather, they form duplexes. Nevertheless, these unintended structures have contributed greatly to the understanding of RNA structure. In a similar occurrence, the homodimer r(GGUCACAGCCC)(2) has been crystallized from an 11-mer/12-mer heteroduplex, r(GGCUGAAGUCCG)/r(GGUCACAGCCC). This surprising phenomenon was observed under a variety of crystallization conditions. The structure of the homoduplex was determined from crystals that differed in the precipitant used and the type of metal present. In all cases, the resulting homoduplexes contain ten base pairings, of which the central six are non-canonical pairings. In two of the variants, ordered metal-binding sites were observed: two equivalent octacoordinate Tl(+) sites in one and two equivalent nanocoordinate Ba(2+) sites in another.
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| - | | + | |
| - | ==About this Structure==
| + | |
| - | 1KD4 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KD4 OCA].
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| - | | + | |
| - | ==Reference==
| + | |
| - | Crystal structures of r(GGUCACAGCCC)2., Kacer V, Scaringe SA, Scarsdale JN, Rife JP, Acta Crystallogr D Biol Crystallogr. 2003 Mar;59(Pt 3):423-32. Epub 2003, Feb 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12595698 12595698]
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| - | [[Category: Protein complex]] | + | |
| - | [[Category: Kacer, V.]] | + | |
| - | [[Category: Rife, J P.]] | + | |
| - | [[Category: Scaringe, S A.]] | + | |
| - | [[Category: Scarsdale, J N.]] | + | |
| - | [[Category: metal binding]]
| + | |
| - | [[Category: rna duplex]]
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| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:46:52 2008''
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