1g0d

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[[Image:1g0d.gif|left|200px]]<br /><applet load="1g0d" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1g0d, resolution 2.5&Aring;" />
 
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'''CRYSTAL STRUCTURE OF RED SEA BREAM TRANSGLUTAMINASE'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF RED SEA BREAM TRANSGLUTAMINASE==
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The crystal structure of the tissue-type transglutaminase from red sea bream liver (fish-derived transglutaminase, FTG) has been determined at 2.5-A resolution using the molecular replacement method, based on the crystal structure of human blood coagulation factor XIII, which is a transglutaminase zymogen. The model contains 666 residues of a total of 695 residues, 382 water molecules, and 1 sulfate ion. FTG consists of four domains, and its overall and active site structures are similar to those of human factor XIII. However, significant structural differences are observed in both the acyl donor and acyl acceptor binding sites, which account for the difference in substrate preferences. The active site of the enzyme is inaccessible to the solvent, because the catalytic Cys-272 hydrogen-bonds to Tyr-515, which is thought to be displaced upon acyl donor binding to FTG. It is postulated that the binding of an inappropriate substrate to FTG would lead to inactivation of the enzyme because of the formation of a new disulfide bridge between Cys-272 and the adjacent Cys-333 immediately after the displacement of Tyr-515. Considering the mutational studies previously reported on the tissue-type transglutaminases, we propose that Cys-333 and Tyr-515 are important in strictly controlling the enzymatic activity of FTG.
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<StructureSection load='1g0d' size='340' side='right'caption='[[1g0d]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1g0d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pagrus_major Pagrus major]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G0D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G0D FirstGlance]. <br>
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1G0D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pagrus_major Pagrus major] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Protein-glutamine_gamma-glutamyltransferase Protein-glutamine gamma-glutamyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.2.13 2.3.2.13] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G0D OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g0d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g0d OCA], [https://pdbe.org/1g0d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g0d RCSB], [https://www.ebi.ac.uk/pdbsum/1g0d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g0d ProSAT]</span></td></tr>
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Crystal structure of red sea bream transglutaminase., Noguchi K, Ishikawa K, Yokoyama Ki, Ohtsuka T, Nio N, Suzuki E, J Biol Chem. 2001 Apr 13;276(15):12055-9. Epub 2000 Nov 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11080504 11080504]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TGM2_PAGMA TGM2_PAGMA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g0/1g0d_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g0d ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Pagrus major]]
[[Category: Pagrus major]]
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[[Category: Protein-glutamine gamma-glutamyltransferase]]
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[[Category: Ishikawa K]]
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[[Category: Single protein]]
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[[Category: Nio N]]
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[[Category: Ishikawa, K.]]
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[[Category: Noguchi K]]
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[[Category: Nio, N.]]
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[[Category: Ohtsuka T]]
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[[Category: Noguchi, K.]]
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[[Category: Suzuki E]]
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[[Category: Ohtsuka, T.]]
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[[Category: Yokoyama K]]
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[[Category: Suzuki, E.]]
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[[Category: Yokoyama, K.]]
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[[Category: SO4]]
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[[Category: acyltransferase]]
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[[Category: tissue transglutaminase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:44:35 2008''
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Current revision

CRYSTAL STRUCTURE OF RED SEA BREAM TRANSGLUTAMINASE

PDB ID 1g0d

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