2kpv

<StructureSection load='2kpv' size='340' side='right' caption='[[2kpv]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>

<table><tr><td colspan='2'>[[2kpv]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KPV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KPV FirstGlance]. <br>

</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kpv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kpv OCA], [http://pdbe.org/2kpv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kpv RCSB], [http://www.ebi.ac.uk/pdbsum/2kpv PDBsum]</span></td></tr>

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== Publication Abstract from PubMed ==

We have determined the 3D structure of a 34-nt RNA construct, herein named LCS1co, which mimics the interaction of let-7 microRNA (miRNA) to one of its complementary binding sites, LCS1, in the 3'-untranslated region of lin-41 mRNA by solution-state NMR spectroscopy. let-7 miRNAs control the timing of development of the nematode Caenorhabditis elegans and are highly conserved in mammals. The sequence and structure of the two conserved let-7 complementary sites, LCS1 and LCS2, in the 3'-untranslated region of lin-41 mRNA are important for a proper downregulation of lin-41. The high-resolution NMR structure reveals details of the binding of let-7 miRNA to lin-41 mRNA which involves formation of a complex with non-canonical structural elements within the seed region. LCS1co exhibits a stem-loop structure with two stems, an asymmetric internal loop and an adenine bulge. Comparison with the NMR solution-state structure of the let-7:lin-41 complex involving the LCS2-binding site shows that conformational freedom of the asymmetric internal loop of LCS1co correlates with a smaller bend between the upper and lower stems in comparison to the well-defined asymmetric loop of LCS2co.

NMR structure of the let-7 miRNA interacting with the site LCS1 of lin-41 mRNA from Caenorhabditis elegans.,Cevec M, Thibaudeau C, Plavec J Nucleic Acids Res. 2010 Nov;38(21):7814-21. Epub 2010 Jul 26. PMID:20660479<ref>PMID:20660479</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 2kpv" style="background-color:#fffaf0;"></div>

== References ==

<references/>

[[Category: Cevec, M]]

[[Category: Plavec, J]]

[[Category: Thibaudeau, C]]

[[Category: Adenine bulge]]

[[Category: Stem-loop]]