2wv6

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{{Seed}}
 
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[[Image:2wv6.jpg|left|200px]]
 
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==Crystal structure of the cholera toxin-like B-subunit from Citrobacter freundii to 1.9 angstrom==
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The line below this paragraph, containing "STRUCTURE_2wv6", creates the "Structure Box" on the page.
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<StructureSection load='2wv6' size='340' side='right'caption='[[2wv6]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2wv6]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Citrobacter_freundii Citrobacter freundii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WV6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WV6 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.895&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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{{STRUCTURE_2wv6| PDB=2wv6 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wv6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wv6 OCA], [https://pdbe.org/2wv6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wv6 RCSB], [https://www.ebi.ac.uk/pdbsum/2wv6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wv6 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q8GAV5_CITFR Q8GAV5_CITFR]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wv/2wv6_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wv6 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Enterotoxigenic Escherichia coli and Vibrio cholerae are well known causative agents of severe diarrheal diseases. Both pathogens produce AB(5) toxins, with one enzymatically active A-subunit and a pentamer of receptor-binding B-subunits. The primary receptor for both B-subunits is the GM1 ganglioside (Galbeta3GalNAcbeta4(NeuAcalpha3)Galbeta4GlcbetaCer), but the B-subunits from porcine isolates of E. coli also bind neolacto-(Galbeta4GlcNAcbeta-)terminated glycoconjugates and the B-subunits from human isolates of E. coli (hLTB) have affinity for blood group A type 2-(GalNAcalpha3(Fucalpha2)Galbeta4GlcNAcbeta-)terminated glycoconjugates. A B-subunit with 73% sequence identity to the B-subunits of cholera toxin and the heat-labile toxin of E. coli is produced by certain strains of enteropathogenic E. coli and by Citrobacter freundii. This C. freundii B-subunit (CFXB) has now been expressed in V. cholerae, and isolated in high yields. Glycosphingolipid binding studies show that CFXB binds to the GM1 ganglioside with high affinity. In addition, CFXB has high affinity for both neolacto-terminated and blood group A type 2-terminated glycoconjugates. The crystal structure of the pentameric arrangement of C. freundii B-subunits display high structural similarity with related proteins from E. coli and V. cholerae and oligosaccharide binding sites can be identified on the protein surface. Small changes in the 88-95 loop connecting the GM1 and blood group A binding sites explains the minor changes in affinity seen for these two ligands. However, the enhanced affinity of CFXB for neolacto-terminated structures can be sought in the Lys34Tyr substitution affording additional hydrogen bond interactions between the tyrosyl side chain and the GlcNAcbeta3Galb4Glcbeta1 segment of neolactotetraosylceramide via bridging water molecules.
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===CRYSTAL STRUCTURE OF THE CHOLERA TOXIN-LIKE B-SUBUNIT FROM CITROBACTER FREUNDII TO 1.9 ANGSTROM===
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Carbohydrate binding specificities and crystal structure of the cholera toxin-like B-subunit from Citrobacter freundii.,Jansson L, Angstrom J, Lebens M, Imberty A, Varrot A, Teneberg S Biochimie. 2010 May;92(5):482-90. Epub 2010 Feb 18. PMID:20171259<ref>PMID:20171259</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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2WV6 is a 10 chains structure of sequences from [http://en.wikipedia.org/wiki/Citrobacter_freundii Citrobacter freundii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WV6 OCA].
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<div class="pdbe-citations 2wv6" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Citrobacter freundii]]
[[Category: Citrobacter freundii]]
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[[Category: Imberty, A.]]
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[[Category: Large Structures]]
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[[Category: Jansson, L.]]
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[[Category: Imberty A]]
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[[Category: Lebens, M.]]
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[[Category: Jansson L]]
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[[Category: Teneberg, S.]]
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[[Category: Lebens M]]
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[[Category: Varrot, A.]]
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[[Category: Teneberg S]]
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[[Category: B subunit]]
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[[Category: Varrot A]]
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[[Category: Cholera toxin-like]]
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[[Category: Lectin]]
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[[Category: Toxin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 28 13:44:05 2009''
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Current revision

Crystal structure of the cholera toxin-like B-subunit from Citrobacter freundii to 1.9 angstrom

PDB ID 2wv6

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