2fid

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[[Image:2fid.gif|left|200px]]
[[Image:2fid.gif|left|200px]]
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{{Structure
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|PDB= 2fid |SIZE=350|CAPTION= <scene name='initialview01'>2fid</scene>, resolution 2.80&Aring;
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The line below this paragraph, containing "STRUCTURE_2fid", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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|GENE= RABGEF1, RABEX5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 Bos taurus])
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{{STRUCTURE_2fid| PDB=2fid | SCENE= }}
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|RELATEDENTRY=[[2fif|2FIF]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fid FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fid OCA], [http://www.ebi.ac.uk/pdbsum/2fid PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2fid RCSB]</span>
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'''Crystal Structure of a Bovine Rabex-5 fragment complexed with ubiquitin'''
'''Crystal Structure of a Bovine Rabex-5 fragment complexed with ubiquitin'''
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[[Category: Hurley, J H.]]
[[Category: Hurley, J H.]]
[[Category: Lee, S.]]
[[Category: Lee, S.]]
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[[Category: helix]]
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[[Category: Helix]]
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[[Category: zinc finger]]
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[[Category: Zinc finger]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:56:02 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:03:10 2008''
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Revision as of 00:56, 4 May 2008

Image:2fid.gif

Template:STRUCTURE 2fid

Crystal Structure of a Bovine Rabex-5 fragment complexed with ubiquitin


Overview

Rabex-5 is an exchange factor for Rab5, a master regulator of endosomal trafficking. Rabex-5 binds monoubiquitin, undergoes covalent ubiquitination and contains an intrinsic ubiquitin ligase activity, all of which require an N-terminal A20 zinc finger followed immediately by a helix. The structure of the N-terminal portion of Rabex-5 bound to ubiquitin at 2.5-A resolution shows that Rabex-5-ubiquitin interactions occur at two sites. The first site is a new type of ubiquitin-binding domain, an inverted ubiquitin-interacting motif, which binds with approximately 29-microM affinity to the canonical Ile44 hydrophobic patch on ubiquitin. The second is a diaromatic patch on the A20 zinc finger, which binds with approximately 22-microM affinity to a polar region centered on Asp58 of ubiquitin. The A20 zinc-finger diaromatic patch mediates ubiquitin-ligase activity by directly recruiting a ubiquitin-loaded ubiquitin-conjugating enzyme.

About this Structure

2FID is a Protein complex structure of sequences from Bos taurus. Full crystallographic information is available from OCA.

Reference

Structural basis for ubiquitin recognition and autoubiquitination by Rabex-5., Lee S, Tsai YC, Mattera R, Smith WJ, Kostelansky MS, Weissman AM, Bonifacino JS, Hurley JH, Nat Struct Mol Biol. 2006 Mar;13(3):264-71. Epub 2006 Feb 5. PMID:16462746 Page seeded by OCA on Sun May 4 03:56:02 2008

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