1v81

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{{STRUCTURE_1v81| PDB=1v81 | SCENE= }}
{{STRUCTURE_1v81| PDB=1v81 | SCENE= }}
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'''Solution structures of ubiquitin at 30 bar and 3 kbar'''
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===Solution structures of ubiquitin at 30 bar and 3 kbar===
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==Overview==
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Conformational fluctuation plays a key role in protein function, but we know little about the associated structural changes. Here we present a general method for elucidating, at the atomic level, a large-scale shape change of a protein molecule in solution undergoing conformational fluctuation. The method utilizes the intimate relationship between conformation and partial molar volume and determines three-dimensional structures of a protein at different pressures using variable pressure NMR technique, whereby NOE distance and torsion angle constraints are used to create average coordinates. Ubiquitin (pH 4.6 at 20 degrees C) was chosen as the first target, for which structures were determined at 30 bar and at 3 kbar, giving "NMR snapshots" of a fluctuating protein structure at atomic resolution. The result reveals that the helix swings in and out by &gt;3 angstroms with a simultaneous reorientation of the C-terminal segment, providing an "open" conformer suitable for enzyme recognition. Spin relaxation analysis indicates that this fluctuation occurs in the ten microsecond time range with activation volumes -4.2(+/-3.2) and 18.5(+/-3.0) ml/mol for the "closed-to-open" and the "open-to-closed" transitions, respectively.
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(as it appears on PubMed at http://www.pubmed.gov), where 15740740 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15740740}}
==About this Structure==
==About this Structure==
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1V81 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V81 OCA].
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1V81 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V81 OCA].
==Reference==
==Reference==
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[[Category: Signaling protein]]
[[Category: Signaling protein]]
[[Category: Structural genomic]]
[[Category: Structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 12:11:52 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 06:13:37 2008''

Revision as of 03:13, 28 July 2008

Image:1v81.png

Template:STRUCTURE 1v81

Solution structures of ubiquitin at 30 bar and 3 kbar

Template:ABSTRACT PUBMED 15740740

About this Structure

1V81 is a Single protein structure of sequence from Bos taurus. Full experimental information is available from OCA.

Reference

NMR snapshots of a fluctuating protein structure: ubiquitin at 30 bar-3 kbar., Kitahara R, Yokoyama S, Akasaka K, J Mol Biol. 2005 Mar 25;347(2):277-85. PMID:15740740

Page seeded by OCA on Mon Jul 28 06:13:37 2008

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