2npm

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[[Image:2npm.png|left|200px]]
 
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==crystal structure of Cryptosporidium parvum 14-3-3 protein in complex with peptide==
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The line below this paragraph, containing "STRUCTURE_2npm", creates the "Structure Box" on the page.
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<StructureSection load='2npm' size='340' side='right'caption='[[2npm]], [[Resolution|resolution]] 2.52&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2npm]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Cryptosporidium_parvum Cryptosporidium parvum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NPM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NPM FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.52&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
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{{STRUCTURE_2npm| PDB=2npm | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2npm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2npm OCA], [https://pdbe.org/2npm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2npm RCSB], [https://www.ebi.ac.uk/pdbsum/2npm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2npm ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q5CUW0_CRYPI Q5CUW0_CRYPI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/np/2npm_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2npm ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The parasite Cryptosporidium parvum has three 14-3-3 proteins: Cp14epsilon, Cp14a and Cp14b, with only Cp14epsilon similar to human 14-3-3 proteins in sequence, peptide-binding properties and structure. Structurally, Cp14a features the classical 14-3-3 dimer but with a uniquely wide pocket and a disoriented RRY triad potentially incapable of binding phosphopeptides. The Cp14b protein deviates from the norm significantly: (i) In one subunit, the phosphorylated C-terminal tail is bound in the binding groove like a phosphopeptide. This supports our binding study indicating this protein was stabilized by a peptide mimicking its last six residues. (ii) The other subunit has eight helices instead of nine, with alphaA and alphaB forming a single helix and occluding the peptide-binding cleft. (iii) The protein forms a degenerate dimer with the two binding grooves divided and facing opposite directions. These features conspire to block and disrupt the bicameral substrate-binding pocket, suggesting a possible tripartite auto-regulation mechanism that has not been observed previously. ENHANCED VERSION: This article can also be viewed as an enhanced version in which the text of the article is integrated with interactive 3D representations and animated transitions. Please note that a web plugin is required to access this enhanced functionality. Instructions for the installation and use of the web plugin are available in Text S1.
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===crystal structure of Cryptosporidium parvum 14-3-3 protein in complex with peptide===
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Characterization of 14-3-3 proteins from Cryptosporidium parvum.,Brokx SJ, Wernimont AK, Dong A, Wasney GA, Lin YH, Lew J, Vedadi M, Lee WH, Hui R PLoS One. 2011;6(8):e14827. Epub 2011 Aug 11. PMID:21853016<ref>PMID:21853016</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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2NPM is a 4 chains structure with sequences from [http://en.wikipedia.org/wiki/Cryptosporidium_parvum Cryptosporidium parvum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NPM OCA].
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<div class="pdbe-citations 2npm" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Cryptosporidium parvum]]
[[Category: Cryptosporidium parvum]]
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[[Category: Arrowsmith, C H.]]
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[[Category: Large Structures]]
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[[Category: Bochkarev, A.]]
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[[Category: Arrowsmith CH]]
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[[Category: Brokx, S.]]
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[[Category: Bochkarev A]]
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[[Category: Dong, A.]]
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[[Category: Brokx S]]
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[[Category: Doyle, D.]]
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[[Category: Dong A]]
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[[Category: Edwards, A M.]]
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[[Category: Doyle D]]
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[[Category: Hassanali, A.]]
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[[Category: Edwards AM]]
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[[Category: Hui, R.]]
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[[Category: Hassanali A]]
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[[Category: Koeieradzki, I.]]
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[[Category: Hui R]]
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[[Category: Lew, J.]]
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[[Category: Koeieradzki I]]
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[[Category: Lin, L.]]
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[[Category: Lew J]]
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[[Category: Qiu, W.]]
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[[Category: Lin L]]
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[[Category: Ren, H.]]
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[[Category: Qiu W]]
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[[Category: SGC, Structural Genomics Consortium.]]
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[[Category: Ren H]]
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[[Category: Sundstrom, M.]]
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[[Category: Sundstrom M]]
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[[Category: Vedadi, M.]]
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[[Category: Vedadi M]]
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[[Category: Wasney, G.]]
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[[Category: Wasney G]]
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[[Category: Weigelt, J.]]
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[[Category: Weigelt J]]
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[[Category: Zhao, Y.]]
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[[Category: Zhao Y]]
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[[Category: Cell regulator protein 14-3-3]]
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[[Category: Cryptosporidium parvum]]
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[[Category: Protein binding]]
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[[Category: Sgc]]
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[[Category: Structural genomic]]
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[[Category: Structural genomics consortium]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 6 14:23:24 2010''
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Current revision

crystal structure of Cryptosporidium parvum 14-3-3 protein in complex with peptide

PDB ID 2npm

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