|
|
| (4 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| | + | |
| | ==NMR STRUCTURE OF THE MU BACTERIOPHAGE REPRESSOR DNA-BINDING DOMAIN== | | ==NMR STRUCTURE OF THE MU BACTERIOPHAGE REPRESSOR DNA-BINDING DOMAIN== |
| - | <StructureSection load='1qpm' size='340' side='right' caption='[[1qpm]], [[NMR_Ensembles_of_Models | 26 NMR models]]' scene=''> | + | <StructureSection load='1qpm' size='340' side='right'caption='[[1qpm]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1qpm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpmu Bpmu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QPM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QPM FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1qpm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_Mu Escherichia virus Mu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QPM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QPM FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qpm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qpm OCA], [http://pdbe.org/1qpm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qpm RCSB], [http://www.ebi.ac.uk/pdbsum/1qpm PDBsum]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qpm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qpm OCA], [https://pdbe.org/1qpm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qpm RCSB], [https://www.ebi.ac.uk/pdbsum/1qpm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qpm ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/REPC_BPMU REPC_BPMU] Promotes latency by binding operators O1 and O2 in the enhancer/operator region, thereby repressing the transcription from the Pe (early) promoter and blocking the expression of the genes required for replication (lytic growth). Competes with DDE-recombinase A for binding to the internal activation sequence (IAS), which overlaps O1 and O2. The outcome of this competition determines if the virus enters latency or starts replication. Makes the cell immune to superinfection by repressing genes expression of any subsequent incoming viral genome.<ref>PMID:11517307</ref> <ref>PMID:12217693</ref> <ref>PMID:16154589</ref> <ref>PMID:18230617</ref> <ref>PMID:8626285</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
| | Check<jmol> | | Check<jmol> |
| | <jmolCheckbox> | | <jmolCheckbox> |
| - | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qp/1qpm_consurf.spt"</scriptWhenChecked> | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qp/1qpm_consurf.spt"</scriptWhenChecked> |
| | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> | | <text>to colour the structure by Evolutionary Conservation</text> |
| Line 24: |
Line 28: |
| | </div> | | </div> |
| | <div class="pdbe-citations 1qpm" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 1qpm" style="background-color:#fffaf0;"></div> |
| - | | |
| - | ==See Also== | |
| - | *[[Bacteriophage repressor|Bacteriophage repressor]] | |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bpmu]] | + | [[Category: Escherichia virus Mu]] |
| - | [[Category: Clubb, R T]] | + | [[Category: Large Structures]] |
| - | [[Category: Connolly, K M]] | + | [[Category: Clubb RT]] |
| - | [[Category: Ilangovan, U]] | + | [[Category: Connolly KM]] |
| - | [[Category: Wojciak, J M]] | + | [[Category: Ilangovan U]] |
| - | [[Category: Helix-turn-helix]] | + | [[Category: Wojciak JM]] |
| - | [[Category: Mu bacteriophage]]
| + | |
| - | [[Category: Repressor]]
| + | |
| - | [[Category: Viral protein]]
| + | |
| Structural highlights
Function
REPC_BPMU Promotes latency by binding operators O1 and O2 in the enhancer/operator region, thereby repressing the transcription from the Pe (early) promoter and blocking the expression of the genes required for replication (lytic growth). Competes with DDE-recombinase A for binding to the internal activation sequence (IAS), which overlaps O1 and O2. The outcome of this competition determines if the virus enters latency or starts replication. Makes the cell immune to superinfection by repressing genes expression of any subsequent incoming viral genome.[1] [2] [3] [4] [5]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The repressor protein of bacteriophage Mu establishes and maintains lysogeny by shutting down transposition functions needed for phage DNA replication. It interacts with several repeated DNA sequences within the early operator, preventing transcription from two divergent promoters. It also directly represses transposition by competing with the MuA transposase for an internal activation sequence (IAS) that is coincident with the operator and required for efficient transposition. The transposase and repressor proteins compete for the operator/IAS region using homologous DNA-binding domains located at their amino termini. Here we present the solution structure of the amino-terminal DNA-binding domain from the repressor protein determined by heteronuclear multidimensional nuclear magnetic resonance spectroscopy. The structure of the repressor DNA-binding domain provides insights into the molecular basis of several temperature sensitive mutations and, in combination with complementary experiments using flourescence anisotropy, surface plasmon resonance, and circular dichroism, defines the structural and biochemical differences between the transposase and repressor DNA-binding modules. We find that the repressor and enhancer domains possess similar three-dimensional structures, thermostabilities, and intrinsic affinities for DNA. This latter result suggests that the higher affinity of the full-length repressor relative to that of the MuA transposase protein originates from cooperative interactions between repressor protomers and not from intrinsic differences in their DNA-binding domains. In addition, we present the results of nucleotide and amino acid mutagenesis which delimits the minimal repressor DNA-binding module and coarsely defines the nucleotide dependence of repressor binding.
NMR structure and functional studies of the Mu repressor DNA-binding domain.,Ilangovan U, Wojciak JM, Connolly KM, Clubb RT Biochemistry. 1999 Jun 29;38(26):8367-76. PMID:10387082[6]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ranquet C, Geiselmann J, Toussaint A. The tRNA function of SsrA contributes to controlling repression of bacteriophage Mu prophage. Proc Natl Acad Sci U S A. 2001 Aug 28;98(18):10220-5. PMID:11517307 doi:10.1073/pnas.171620598
- ↑ O'Handley D, Nakai H. Derepression of bacteriophage mu transposition functions by truncated forms of the immunity repressor. J Mol Biol. 2002 Sep 13;322(2):311-24. PMID:12217693 doi:10.1016/s0022-2836(02)00755-6
- ↑ Ranquet C, Toussaint A, de Jong H, Maenhaut-Michel G, Geiselmann J. Control of bacteriophage mu lysogenic repression. J Mol Biol. 2005 Oct 14;353(1):186-95. PMID:16154589 doi:10.1016/j.jmb.2005.08.015
- ↑ Marshall-Batty KR, Nakai H. Activation of a dormant ClpX recognition motif of bacteriophage Mu repressor by inducing high local flexibility. J Biol Chem. 2008 Apr 4;283(14):9060-70. PMID:18230617 doi:10.1074/jbc.M705508200
- ↑ Kahmeyer-Gabbe M, Howe MM. Regulatory factors acting at the bacteriophage Mu middle promoter. J Bacteriol. 1996 Mar;178(6):1585-92. PMID:8626285 doi:10.1128/jb.178.6.1585-1592.1996
- ↑ Ilangovan U, Wojciak JM, Connolly KM, Clubb RT. NMR structure and functional studies of the Mu repressor DNA-binding domain. Biochemistry. 1999 Jun 29;38(26):8367-76. PMID:10387082 doi:10.1021/bi990530b
|
