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| | ==Bacterial chalcone isomerase in closed conformation from Eubacterium ramulus at 2.8 A resolution== | | ==Bacterial chalcone isomerase in closed conformation from Eubacterium ramulus at 2.8 A resolution== |
| - | <StructureSection load='3zph' size='340' side='right' caption='[[3zph]], [[Resolution|resolution]] 2.80Å' scene=''> | + | <StructureSection load='3zph' size='340' side='right'caption='[[3zph]], [[Resolution|resolution]] 2.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3zph]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_29099 Atcc 29099]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZPH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZPH FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3zph]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Eubacterium_ramulus Eubacterium ramulus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZPH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZPH FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chalcone_isomerase Chalcone isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.5.1.6 5.5.1.6] </span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zph FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zph OCA], [http://pdbe.org/3zph PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3zph RCSB], [http://www.ebi.ac.uk/pdbsum/3zph PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3zph ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zph FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zph OCA], [https://pdbe.org/3zph PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zph RCSB], [https://www.ebi.ac.uk/pdbsum/3zph PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zph ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/U2Q8X2_EUBRA U2Q8X2_EUBRA] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 29099]] | + | [[Category: Eubacterium ramulus]] |
| - | [[Category: Chalcone isomerase]] | + | [[Category: Large Structures]] |
| - | [[Category: Hinrichs, W]] | + | [[Category: Hinrichs W]] |
| - | [[Category: Palm, G J]] | + | [[Category: Palm GJ]] |
| - | [[Category: Thomsen, M]] | + | [[Category: Thomsen M]] |
| - | [[Category: Flavonoid degradation]]
| + | |
| - | [[Category: Isomerase]]
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| Structural highlights
Function
U2Q8X2_EUBRA
Publication Abstract from PubMed
Flavonoids are a large group of plant secondary metabolites with a variety of biological properties and are therefore of interest to many scientists, as they can lead to industrially interesting intermediates. The anaerobic gut bacterium Eubacterium ramulus can catabolize flavonoids, but until now, the pathway has not been experimentally confirmed. In the present work, a chalcone isomerase (CHI) and an enoate reductase (ERED) could be identified through whole genome sequencing and gene motif search. These two enzymes were successfully cloned and expressed in Escherichia coli in their active form, even under aerobic conditions. The catabolic pathway of E. ramulus was confirmed by biotransformations of flavanones into dihydrochalcones. The engineered E. coli strain that expresses both enzymes was used for the conversion of several flavanones, underlining the applicability of this biocatalytic cascade reaction.
Enzymatic Conversion of Flavonoids using Bacterial Chalcone Isomerase and Enoate Reductase.,Gall M, Thomsen M, Peters C, Pavlidis IV, Jonczyk P, Grunert PP, Beutel S, Scheper T, Gross E, Backes M, Geissler T, Ley JP, Hilmer JM, Krammer G, Palm GJ, Hinrichs W, Bornscheuer UT Angew Chem Int Ed Engl. 2014 Jan 27;53(5):1439-42. doi: 10.1002/anie.201306952., Epub 2013 Dec 20. PMID:24459060[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Gall M, Thomsen M, Peters C, Pavlidis IV, Jonczyk P, Grunert PP, Beutel S, Scheper T, Gross E, Backes M, Geissler T, Ley JP, Hilmer JM, Krammer G, Palm GJ, Hinrichs W, Bornscheuer UT. Enzymatic Conversion of Flavonoids using Bacterial Chalcone Isomerase and Enoate Reductase. Angew Chem Int Ed Engl. 2014 Jan 27;53(5):1439-42. doi: 10.1002/anie.201306952., Epub 2013 Dec 20. PMID:24459060 doi:http://dx.doi.org/10.1002/anie.201306952
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