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| | <StructureSection load='5tcs' size='340' side='right'caption='[[5tcs]], [[Resolution|resolution]] 2.83Å' scene=''> | | <StructureSection load='5tcs' size='340' side='right'caption='[[5tcs]], [[Resolution|resolution]] 2.83Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5tcs]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TCS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TCS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5tcs]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TCS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5TCS FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8313Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NDC80, HEC1, TID3, YIL144W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), NUF2, YOL069W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), SPC24, YMR117C, YM9718.16C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), SPC25, YER018C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5tcs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tcs OCA], [https://pdbe.org/5tcs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5tcs RCSB], [https://www.ebi.ac.uk/pdbsum/5tcs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5tcs ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5tcs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tcs OCA], [http://pdbe.org/5tcs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5tcs RCSB], [http://www.ebi.ac.uk/pdbsum/5tcs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5tcs ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SPC24_YEAST SPC24_YEAST]] Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.<ref>PMID:11266451</ref> <ref>PMID:11952896</ref> <ref>PMID:12514103</ref> [[http://www.uniprot.org/uniprot/NUF2_YEAST NUF2_YEAST]] Acts as component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.<ref>PMID:11266451</ref> <ref>PMID:12514103</ref> [[http://www.uniprot.org/uniprot/NDC80_YEAST NDC80_YEAST]] Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.<ref>PMID:10409732</ref> <ref>PMID:11266451</ref> <ref>PMID:12514103</ref> <ref>PMID:9585415</ref> [[http://www.uniprot.org/uniprot/SPC25_YEAST SPC25_YEAST]] Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.<ref>PMID:11266451</ref> <ref>PMID:12514103</ref> | + | [https://www.uniprot.org/uniprot/NUF2_YEAST NUF2_YEAST] Acts as component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.<ref>PMID:11266451</ref> <ref>PMID:12514103</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Baker's yeast]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Harrison, S C]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Valverde, R]] | + | [[Category: Harrison SC]] |
| - | [[Category: Ch]] | + | [[Category: Valverde R]] |
| - | [[Category: Coiled-coil]]
| + | |
| - | [[Category: Kinetochore]]
| + | |
| - | [[Category: Ndc80]]
| + | |
| - | [[Category: Replication]]
| + | |
| - | [[Category: Rwd]]
| + | |
| - | [[Category: Tetramer]]
| + | |
| Structural highlights
Function
NUF2_YEAST Acts as component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.[1] [2]
Publication Abstract from PubMed
The heterotetrameric Ndc80 complex establishes connectivity along the principal longitudinal axis of a kinetochore. Its two heterodimeric subcomplexes, each with a globular end and a coiled-coil shaft, connect end-to-end to create a approximately 600 A long rod spanning the gap from centromere-proximal structures to spindle microtubules. Neither subcomplex has a known function on its own, but the heterotetrameric organization and the characteristics of the junction are conserved from yeast to man. We have determined crystal structures of two shortened ("dwarf") Ndc80 complexes that contain the full tetramer junction and both globular ends. The junction connects two alpha-helical coiled coils through regions of four-chain and three-chain overlap. The complexity of its structure depends on interactions among conserved amino-acid residues, suggesting a binding site for additional cellular factor(s) not yet identified.
Conserved Tetramer Junction in the Kinetochore Ndc80 Complex.,Valverde R, Ingram J, Harrison SC Cell Rep. 2016 Nov 15;17(8):1915-1922. doi: 10.1016/j.celrep.2016.10.065. PMID:27851957[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Wigge PA, Kilmartin JV. The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation. J Cell Biol. 2001 Jan 22;152(2):349-60. PMID:11266451
- ↑ McCleland ML, Gardner RD, Kallio MJ, Daum JR, Gorbsky GJ, Burke DJ, Stukenberg PT. The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity. Genes Dev. 2003 Jan 1;17(1):101-14. PMID:12514103 doi:http://dx.doi.org/10.1101/gad.1040903
- ↑ Valverde R, Ingram J, Harrison SC. Conserved Tetramer Junction in the Kinetochore Ndc80 Complex. Cell Rep. 2016 Nov 15;17(8):1915-1922. doi: 10.1016/j.celrep.2016.10.065. PMID:27851957 doi:http://dx.doi.org/10.1016/j.celrep.2016.10.065
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