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| | <StructureSection load='3f4c' size='340' side='right'caption='[[3f4c]], [[Resolution|resolution]] 2.07Å' scene=''> | | <StructureSection load='3f4c' size='340' side='right'caption='[[3f4c]], [[Resolution|resolution]] 2.07Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3f4c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_stearothermophilus_10 Bacillus stearothermophilus 10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F4C OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3F4C FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3f4c]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus_10 Geobacillus stearothermophilus 10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F4C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3F4C FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.07Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3f4d|3f4d]]</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3f4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3f4c OCA], [https://pdbe.org/3f4c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3f4c RCSB], [https://www.ebi.ac.uk/pdbsum/3f4c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3f4c ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3f4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3f4c OCA], [http://pdbe.org/3f4c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3f4c RCSB], [http://www.ebi.ac.uk/pdbsum/3f4c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3f4c ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/D0VX06_GEOS3 D0VX06_GEOS3] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus stearothermophilus 10]] | + | [[Category: Geobacillus stearothermophilus 10]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Aikens, J]] | + | [[Category: Aikens J]] |
| - | [[Category: Hawwa, R]] | + | [[Category: Hawwa R]] |
| - | [[Category: Mesecar, A]] | + | [[Category: Mesecar A]] |
| - | [[Category: Santarsiero, B]] | + | [[Category: Santarsiero B]] |
| - | [[Category: Turner, R J]] | + | [[Category: Turner RJ]] |
| - | [[Category: Alpha-beta barrel]]
| + | |
| - | [[Category: Amidohydrolase]]
| + | |
| - | [[Category: Binuclear metal enzyme]]
| + | |
| - | [[Category: Glycerol-bound]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| Structural highlights
Function
D0VX06_GEOS3
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
A new enzyme homologous to phosphotriesterase was identified from the bacterium Geobacillus stearothermophilus (GsP). This enzyme belongs to the amidohydrolase family and possesses the ability to hydrolyze both lactone and organophosphate (OP) compounds, making it a phosphotriesterase-like lactonase (PLL). GsP possesses higher OP-degrading activity than recently characterized PLLs, and it is extremely thermostable. GsP is active up to 100 degrees C with an energy of activation of 8.0 kcal/mol towards ethyl paraoxon, and it can withstand an incubation temperature of 60 degrees C for two days. In an attempt to understand the thermostability of PLLs, the X-ray structure of GsP was determined and compared to those of existing PLLs. Based upon a comparative analysis, a new thermal advantage score and plot was developed and reveals that a number of different factors contribute to the thermostability of PLLs.
Structural basis for thermostability revealed through the identification and characterization of a highly thermostable phosphotriesterase-like lactonase from Geobacillus stearothermophilus.,Hawwa R, Aikens J, Turner RJ, Santarsiero BD, Mesecar AD Arch Biochem Biophys. 2009 Aug 15;488(2):109-20. Epub 2009 Jul 16. PMID:19615330[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Hawwa R, Aikens J, Turner RJ, Santarsiero BD, Mesecar AD. Structural basis for thermostability revealed through the identification and characterization of a highly thermostable phosphotriesterase-like lactonase from Geobacillus stearothermophilus. Arch Biochem Biophys. 2009 Aug 15;488(2):109-20. Epub 2009 Jul 16. PMID:19615330 doi:10.1016/j.abb.2009.06.005
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