6ytc

<StructureSection load='6ytc' size='340' side='right'caption='[[6ytc]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>

<table><tr><td colspan='2'>[[6ytc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_thetaiotaomicron"_distaso_1912 "bacillus thetaiotaomicron" distaso 1912]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YTC OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6YTC FirstGlance]. <br>

</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HMPREF2534_01945 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=818 "Bacillus thetaiotaomicron" Distaso 1912])</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ytc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ytc OCA], [http://pdbe.org/6ytc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ytc RCSB], [http://www.ebi.ac.uk/pdbsum/6ytc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ytc ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

In Bacteroidetes, one of the dominant phyla of the mammalian gut, active uptake of large nutrients across the outer membrane is mediated by SusCD protein complexes via a "pedal bin" transport mechanism. However, many features of SusCD function in glycan uptake remain unclear, including ligand binding, the role of the SusD lid and the size limit for substrate transport. Here we characterise the beta2,6 fructo-oligosaccharide (FOS) importing SusCD from Bacteroides thetaiotaomicron (Bt1762-Bt1763) to shed light on SusCD function. Co-crystal structures reveal residues involved in glycan recognition and suggest that the large binding cavity can accommodate several substrate molecules, each up to ~2.5 kDa in size, a finding supported by native mass spectrometry and isothermal titration calorimetry. Mutational studies in vivo provide functional insights into the key structural features of the SusCD apparatus and cryo-EM of the intact dimeric SusCD complex reveals several distinct states of the transporter, directly visualising the dynamics of the pedal bin transport mechanism.

Insights into SusCD-mediated glycan import by a prominent gut symbiont.,Gray DA, White JBR, Oluwole AO, Rath P, Glenwright AJ, Mazur A, Zahn M, Basle A, Morland C, Evans SL, Cartmell A, Robinson CV, Hiller S, Ranson NA, Bolam DN, van den Berg B Nat Commun. 2021 Jan 4;12(1):44. doi: 10.1038/s41467-020-20285-y. PMID:33398001<ref>PMID:33398001</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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== References ==

<references/>

[[Category: Bacillus thetaiotaomicron distaso 1912]]

[[Category: Hiller, S]]

[[Category: Mazur, A]]

[[Category: Rath, P]]

[[Category: Transporter]]