2b4l

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(New page: 200px<br /><applet load="2b4l" size="450" color="white" frame="true" align="right" spinBox="true" caption="2b4l, resolution 2.00&Aring;" /> '''Crystal structure of...)
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[[Image:2b4l.gif|left|200px]]<br /><applet load="2b4l" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2b4l, resolution 2.00&Aring;" />
 
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'''Crystal structure of the binding protein OpuAC in complex with glycine betaine'''<br />
 
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==Overview==
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==Crystal structure of the binding protein OpuAC in complex with glycine betaine==
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Compatible solutes play a decisive role in the defense of microorganisms, against changes in temperature and increases in osmolarity in their, natural habitats. In Bacillus subtilis, the substrate-binding protein, (SBP)-dependent ABC-transporter OpuA serves for the uptake of the, compatible solutes glycine betaine (GB) and proline betaine (PB). Here, we, report the determinants of compatible solute binding by OpuAC, the SBP of, the OpuA transporter, by equilibrium binding studies and X-ray, crystallography. The affinity of OpuAC/GB and OpuAC/PB complexes were, analyzed by intrinsic tryptophan fluorescence and the K(D) values were, determined to be 17(+/-1)microM for GB and 295(+/-27)microM for PB, respectively. The structures of OpuAC in complex with GB or PB were solved, at 2.0 A and 2.8 A, respectively, and show an SBP-typical class II fold., The ligand-binding pocket is formed by three tryptophan residues arranged, in a prism-like geometry suitable to coordinate the positive charge of the, trimethyl ammonium group of GB and the dimethyl ammonium group of PB by, cation-pi interactions and by hydrogen bonds with the carboxylate moiety, of the ligand. Structural differences between the OpuAC/GB and OpuAC/PB, complexes occur within the ligand-binding pocket as well as across the, domain-domain interface. These differences provide a structural framework, to explain the drastic differences in affinity of the OpuAC/GB and, OpuAC/PB complexes. A sequence comparison with putative SBP specific for, compatible solutes reveals the presence of three distinct families for, which the crystal structure of OpuAC might serve as a suitable template to, predict the structures of these putative compatible solute-binding, proteins.
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<StructureSection load='2b4l' size='340' side='right'caption='[[2b4l]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2b4l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B4L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B4L FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BET:TRIMETHYL+GLYCINE'>BET</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b4l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b4l OCA], [https://pdbe.org/2b4l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b4l RCSB], [https://www.ebi.ac.uk/pdbsum/2b4l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b4l ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/OPUAC_BACSU OPUAC_BACSU] Involved in a multicomponent binding-protein-dependent transport system for glycine betaine.<ref>PMID:7622480</ref> <ref>PMID:8752321</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b4/2b4l_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2b4l ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2B4L is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with BET and EDO as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2B4L OCA].
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*[[ABC transporter 3D structures|ABC transporter 3D structures]]
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== References ==
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==Reference==
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<references/>
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Molecular determinants for substrate specificity of the ligand-binding protein OpuAC from Bacillus subtilis for the compatible solutes glycine betaine and proline betaine., Horn C, Sohn-Bosser L, Breed J, Welte W, Schmitt L, Bremer E, J Mol Biol. 2006 Mar 24;357(2):592-606. Epub 2006 Jan 13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16445940 16445940]
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__TOC__
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</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Breed, J.]]
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[[Category: Breed J]]
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[[Category: Bremer, E.]]
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[[Category: Bremer E]]
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[[Category: Horn, C.]]
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[[Category: Horn C]]
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[[Category: Schmitt, L.]]
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[[Category: Schmitt L]]
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[[Category: Sohn-Boesser, L.]]
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[[Category: Sohn-Boesser L]]
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[[Category: Welte, W.]]
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[[Category: Welte W]]
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[[Category: BET]]
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[[Category: EDO]]
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[[Category: abc-transporter]]
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[[Category: closed liganded]]
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[[Category: compatible solutes]]
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[[Category: substrate-binding protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 08:33:44 2007''
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Current revision

Crystal structure of the binding protein OpuAC in complex with glycine betaine

PDB ID 2b4l

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