2roh

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{{Seed}}
 
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[[Image:2roh.jpg|left|200px]]
 
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==The DNA binding domain of RTBP1==
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The line below this paragraph, containing "STRUCTURE_2roh", creates the "Structure Box" on the page.
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<StructureSection load='2roh' size='340' side='right'caption='[[2roh]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2roh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa Oryza sativa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ROH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ROH FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2roh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2roh OCA], [https://pdbe.org/2roh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2roh RCSB], [https://www.ebi.ac.uk/pdbsum/2roh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2roh ProSAT]</span></td></tr>
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</table>
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{{STRUCTURE_2roh| PDB=2roh | SCENE= }}
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== Function ==
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[https://www.uniprot.org/uniprot/TBP1_ORYSJ TBP1_ORYSJ] Binds the telomeric double-stranded 5'TTTAGGG-3' repeat and regulates telomere length and structure.<ref>PMID:10811811</ref> <ref>PMID:17586654</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ro/2roh_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2roh ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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RTBP1 is a rice telomeric protein that binds to the duplex array of TTTAGGG repeats at chromosome ends. The DNA binding domain of RTBP1 contains a Myb-type DNA binding motif and a highly conserved C-terminal Myb extension that is unique to plant telomeric proteins. Using an electrophoretic mobility shift assay, we identified the C-terminal 110-amino acid region (RTBP1(506-615)) as the minimal telomeric DNA binding domain, suggesting that the Myb extension is required for binding plant telomeric DNA. Like other telomeric proteins such as human TRF1 and yeast Rap1, RTBP1 induced a DNA bending in the telomeric repeat sequence, suggesting that RTBP1 may play a role in establishing and/or maintaining an active telomere configuration in vivo. To elucidate the DNA binding mode of RTBP1, we determined the three-dimensional structure of RTBP1(506-615) in solution by NMR spectroscopy. The overall structure of RTBP1(506-615) is composed of four alpha-helices and stabilized by three hydrophobic patches. The second and third helices in RTBP1 form a helix-turn-helix motif that interacts directly with DNA. The fourth helix located in the Myb extension is essential for binding to telomeric DNA via stabilization of the overall structure of the RTBP1 DNA binding domain. When DNA bound to RTBP1(506-615), large chemical shift perturbations were induced in the N-terminal arm, helix 3, and the loop between helices 3 and 4. These results suggest that helix 3 functions as a sequence-specific recognition helix while the N-terminal arm stabilizes the DNA binding.
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===The DNA binding domain of RTBP1===
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Solution structure of the DNA binding domain of rice telomere binding protein RTBP1.,Ko S, Yu EY, Shin J, Yoo HH, Tanaka T, Kim WT, Cho HS, Lee W, Chung IK Biochemistry. 2009 Feb 10;48(5):827-38. PMID:19152316<ref>PMID:19152316</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2roh" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19152316 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19152316}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2ROH is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Oryza_sativa Oryza sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ROH OCA].
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==Reference==
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<ref group="xtra">PMID:19152316</ref><references group="xtra"/>
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[[Category: Oryza sativa]]
[[Category: Oryza sativa]]
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[[Category: Ko, S.]]
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[[Category: Ko S]]
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[[Category: Lee, W.]]
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[[Category: Lee W]]
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[[Category: Dna binding protein]]
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[[Category: Nucleus]]
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[[Category: Plant]]
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[[Category: Telomere binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 25 12:09:15 2009''
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Current revision

The DNA binding domain of RTBP1

PDB ID 2roh

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