3f4c

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{{Seed}}
 
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[[Image:3f4c.jpg|left|200px]]
 
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==Crystal structure of organophosphorus hydrolase from Geobacillus stearothermophilus strain 10, with glycerol bound==
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The line below this paragraph, containing "STRUCTURE_3f4c", creates the "Structure Box" on the page.
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<StructureSection load='3f4c' size='340' side='right'caption='[[3f4c]], [[Resolution|resolution]] 2.07&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3f4c]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus_10 Geobacillus stearothermophilus 10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F4C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3F4C FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.07&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr>
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{{STRUCTURE_3f4c| PDB=3f4c | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3f4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3f4c OCA], [https://pdbe.org/3f4c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3f4c RCSB], [https://www.ebi.ac.uk/pdbsum/3f4c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3f4c ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/D0VX06_GEOS3 D0VX06_GEOS3]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f4/3f4c_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3f4c ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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A new enzyme homologous to phosphotriesterase was identified from the bacterium Geobacillus stearothermophilus (GsP). This enzyme belongs to the amidohydrolase family and possesses the ability to hydrolyze both lactone and organophosphate (OP) compounds, making it a phosphotriesterase-like lactonase (PLL). GsP possesses higher OP-degrading activity than recently characterized PLLs, and it is extremely thermostable. GsP is active up to 100 degrees C with an energy of activation of 8.0 kcal/mol towards ethyl paraoxon, and it can withstand an incubation temperature of 60 degrees C for two days. In an attempt to understand the thermostability of PLLs, the X-ray structure of GsP was determined and compared to those of existing PLLs. Based upon a comparative analysis, a new thermal advantage score and plot was developed and reveals that a number of different factors contribute to the thermostability of PLLs.
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===Crystal structure of organophosphorus hydrolase from Geobacillus stearothermophilus strain 10, with glycerol bound===
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Structural basis for thermostability revealed through the identification and characterization of a highly thermostable phosphotriesterase-like lactonase from Geobacillus stearothermophilus.,Hawwa R, Aikens J, Turner RJ, Santarsiero BD, Mesecar AD Arch Biochem Biophys. 2009 Aug 15;488(2):109-20. Epub 2009 Jul 16. PMID:19615330<ref>PMID:19615330</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19615330}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3f4c" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19615330 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19615330}}
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__TOC__
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</StructureSection>
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==About this Structure==
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3F4C is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus_10 Geobacillus stearothermophilus 10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F4C OCA].
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==Reference==
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<ref group="xtra">PMID:19615330</ref><references group="xtra"/>
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[[Category: Geobacillus stearothermophilus 10]]
[[Category: Geobacillus stearothermophilus 10]]
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[[Category: Aikens, J.]]
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[[Category: Large Structures]]
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[[Category: Hawwa, R.]]
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[[Category: Aikens J]]
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[[Category: Mesecar, A.]]
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[[Category: Hawwa R]]
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[[Category: Santarsiero, B.]]
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[[Category: Mesecar A]]
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[[Category: Turner, R J.]]
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[[Category: Santarsiero B]]
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[[Category: Alpha-beta barrel]]
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[[Category: Turner RJ]]
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[[Category: Amidohydrolase]]
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[[Category: Binuclear metal enzyme]]
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[[Category: Glycerol-bound]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 16 08:54:04 2009''
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Current revision

Crystal structure of organophosphorus hydrolase from Geobacillus stearothermophilus strain 10, with glycerol bound

PDB ID 3f4c

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